Department of Zoonoses, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.
Sci Rep. 2024 Sep 18;14(1):21741. doi: 10.1038/s41598-024-71610-0.
Enterococci, common hospital-acquired infections in immunocompromised patients, have garnered attention in clinical microbiology. To determine the clinical relevance of enterococci as food-borne pathogens, 116 fish, 90 vegetables, and 120 human diarrheal samples were tested for E. faecalis and E. faecium pathogenicity. Conventionally, 69 of 326 (21.17%) samples were positive for Enterococcus species, 52 (15.95%) of which were molecularly classified as E. faecalis and 13 (3.99%) as E. faecium. The E. faecalis contamination percentage of fresh fish (19.70%) was higher than frozen fish (4%). Cauliflower had the highest E. faecalis percentage (16.67%) when fish and vegetable samples didn't harbor the E. faecium atpA gene. 23.33% and 10.83% of participants' samples were molecularly confirmed as E. faecalis and E. faecium positive, respectively. E. faecalis isolates had all virulence genes, with gels being the most common (65.38%), while cylA and asa1 genes couldn't be detected in E. faecium isolates. E. faecalis showed the highest resistance against vancomycin and tetracycline (69.23%), whereas E. faecium extremely resisted tetracycline (76.92%) and erythromycin (69.23%) with the recognition of MDR among 44.2% of E. faecalis and 38.5% of E. faecium isolates. The great similarity of our isolates showed the clinical importance of food-borne antibiotic-resistant enterococci.
肠球菌是免疫功能低下患者医院获得性感染的常见病原体，在临床微生物学中受到关注。为了确定肠球菌作为食源性病原体的临床相关性，对 116 份鱼类、90 份蔬菜和 120 份人类腹泻样本进行了粪肠球菌和屎肠球菌致病性检测。传统方法检测，326 份样本中有 69 份（21.17%）为肠球菌属阳性，其中 52 份（15.95%）经分子分类为粪肠球菌，13 份（3.99%）为屎肠球菌。新鲜鱼类中肠球菌的污染率（19.70%）高于冷冻鱼类（4%）。当鱼类和蔬菜样本中不携带屎肠球菌 atpA 基因时，花椰菜的粪肠球菌检出率最高（16.67%）。23.33%和 10.83%的参与者样本经分子确认为粪肠球菌和屎肠球菌阳性。肠球菌分离株均携带所有毒力基因，凝胶酶最为常见（65.38%），而 cylA 和 asa1 基因在屎肠球菌分离株中无法检测到。肠球菌对万古霉素和四环素的耐药率最高（69.23%），而屎肠球菌对四环素（76.92%）和红霉素（69.23%）的耐药性极强，44.2%的肠球菌和 38.5%的屎肠球菌分离株表现出 MDR。我们分离株的高度相似性表明了食源性抗生素耐药肠球菌的临床重要性。
