Department of Pathology, School of Dentistry, Kyungpook National University, Kyungpook National University Hospital, Daegu, Republic of Korea.
Department of Anesthesia and Pain Medicine, School of Dentistry, Institute for Translational Research in Dentistry, Kyungpook National University, Daegu, Republic of Korea.
BMC Oral Health. 2024 Sep 17;24(1):1102. doi: 10.1186/s12903-024-04873-8.
BACKGROUND/PURPOSE: This retrospective immunohistological pilot study aimed to investigate the influence of natural killer group 2, member D (NKG2D) ligand expression on ameloblastoma recurrence after surgical resection. It also aimed to elucidate additional clinical factors that could serve as predictors of ameloblastoma recurrence.
This study included 96 patients who were histologically diagnosed with ameloblastoma after surgical resection. The expression of NKG2D ligands, including UL16-binding proteins (ULBPs) 1-3 and major histocompatibility complex class I chain-related molecule (MIC) A/B, was evaluated in formalin-fixed paraffin-embedded tumor tissues via immunohistochemistry assays. Furthermore, the patients' electronic medical records were reviewed. Multivariate Cox regression analysis was conducted, and data were expressed as adjusted hazard ratios [HRs] with 95% confidence intervals [95% CIs].
Multivariate analysis revealed that recurrent tumors (ref.: primary; adjusted HR [95% CI]: 2.780 [1.136, 6.803], p = 0.025) and positive MICA/B expression (ref.: negative; adjusted HR [95% CI]: 0.223 [0.050, 0.989], p = 0.048) independently affected recurrence-free survival in ameloblastoma.
This study identified recurrent cases and loss of MICA/B expression as independent predictors of early ameloblastoma recurrence following surgical resection. The findings suggest that decreased MICA/B expression might undermine NKG2D-mediated tumor immunosurveillance, thereby influencing early recurrence.
背景/目的：本回顾性免疫组织化学研究旨在探讨自然杀伤细胞组 2，成员 D（NKG2D）配体表达对手术切除后成釉细胞瘤复发的影响。本研究还旨在阐明其他可能作为成釉细胞瘤复发预测因子的临床因素。
本研究纳入了 96 例经手术切除后组织学诊断为成釉细胞瘤的患者。采用免疫组织化学法检测福尔马林固定、石蜡包埋的肿瘤组织中 NKG2D 配体，包括 UL16 结合蛋白（ULBPs）1-3 和主要组织相容性复合体 I 类链相关分子（MIC）A/B 的表达。此外，还回顾了患者的电子病历。采用多变量 Cox 回归分析，数据以调整后的风险比（HR）和 95%置信区间（95%CI）表示。
多变量分析显示，复发性肿瘤（参考：原发性；调整后的 HR [95%CI]：2.780 [1.136, 6.803]，p=0.025）和 MICA/B 阳性表达（参考：阴性；调整后的 HR [95%CI]：0.223 [0.050, 0.989]，p=0.048）独立影响成釉细胞瘤无复发生存率。
本研究确定了复发性病例和 MICA/B 表达缺失是手术切除后早期成釉细胞瘤复发的独立预测因子。这些发现表明，MICA/B 表达的降低可能会破坏 NKG2D 介导的肿瘤免疫监视，从而影响早期复发。
