Fallahi Fariba, Tahmasebi Worya, Rahimi Mohammad Rahman, Azizi Mohammad
Exercise Physiology Department, Faculty of Sport Sciences, Razi University, Kermanshah, Iran.
Exercise Physiology Department, Faculty of Sport Sciences, Razi University, Kermanshah, Iran. Email:
Cell J. 2024 Sep 11;26(7):465-472. doi: 10.22074/cellj.2024.2027582.1560.
The purpose of this study was to investigate the effects of 8 weeks of high-intensity interval training (HIIT) and vitamin D3 supplementation on Chemokine (C-C motif) Ligand 5 (CCL-5) and C-C motif chemokine receptor 5 (CCR5) in the white adipose tissue (WAT) of male rats with type 2 diabetes (T2DM).
The experimental study involved 40 male Wistar rats divided into 5 groups (n=8). These groups were healthy control (HC), diabetic control (DC), diabetic+HIIT (DHIIT), diabetic+vitamin D3 (DD3), and diabetic+HIIT+vitamin D3 (DHIITD3). The rats completed 8 weeks of HIIT, consisting of 12 sessions lasting 1 minute each at an intensity of 90-95% of their maximum running speed. Additionally, the rats were administered a weekly dose of 10,000 IU/kg of vitamin D3 for 8 weeks.
The levels of CCL-5 (P<0.001) and CCR5 (P=0.003) were found to be higher in the DC group as compared to the HC group. However, when HIIT training and vitamin D3 were administered together, there was a decrease in CCL-5 (P=0.001) and CCR5 (P<0.001) in the DHIITD3 group (P=0.001). Similarly, vitamin D3 alone reduced CCR5 levels in the DD3 group (P< 0.001). Also, the decrease of CCR5 in the DD3 group was higher than in the DHIIT group (P=0.022), and the DHIITD3 group was higher than in the DHIIT group (P<0.001), but there was no difference between the DD3 and DHIITD3 groups (P≥0.05).
The results indicate that combining HIIT training with vitamin D3 has a greater effect on reducing the expression of CCL-5 and CCR5 in the white adipose tissue of rats with type 2 diabetes induced by streptozotocin (STZ) and a high-fat diet (HFD), compared to the effects of each one alone. It is recommended that the study be conducted by measuring the variables involved in the mechanisms and the changes in CCL-5 and CCR5.
本研究旨在探讨8周高强度间歇训练（HIIT）和补充维生素D3对链脲佐菌素（STZ）诱导的高脂饮食（HFD）所致2型糖尿病（T2DM）雄性大鼠白色脂肪组织（WAT）中趋化因子（C-C基序）配体5（CCL-5）和C-C基序趋化因子受体5（CCR5）的影响。
实验研究涉及40只雄性Wistar大鼠，分为5组（n = 8）。这些组分别为健康对照组（HC）、糖尿病对照组（DC）、糖尿病+HIIT组（DHIIT）、糖尿病+维生素D3组（DD3）和糖尿病+HIIT+维生素D3组（DHIITD3）。大鼠完成8周的HIIT，包括12节训练课，每节持续1分钟，强度为其最大跑步速度的90 - 95%。此外，大鼠每周接受一次剂量为10,000 IU/kg的维生素D3，持续8周。
发现DC组中CCL-5（P < 0.001）和CCR5（P = 0.003）水平高于HC组。然而，当同时进行HIIT训练和补充维生素D3时，DHIITD3组中CCL-5（P = 0.001）和CCR5（P < 0.001）有所下降（P = 0.001）。同样，单独补充维生素D3可降低DD3组中的CCR5水平（P < 0.001）。此外，DD3组中CCR5的下降高于DHIIT组（P = 0.022），DHIITD3组高于DHIIT组（P < 0.001），但DD3组和DHIITD3组之间无差异（P≥0.05）。
结果表明，与单独进行HIIT训练或单独补充维生素D3相比，将HIIT训练与维生素D3联合使用对降低链脲佐菌素（STZ）和高脂饮食（HFD）诱导的2型糖尿病大鼠白色脂肪组织中CCL-5和CCR5的表达具有更大的作用。建议通过测量参与机制的变量以及CCL-5和CCR5的变化来进行该研究。
