Xie Jinfeng, Liao Yonggui, Wang Dile
Department of Vascular Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Histol Histopathol. 2025 Apr;40(4):547-554. doi: 10.14670/HH-18-799. Epub 2024 Jul 26.
Deep vein thrombosis (DVT) is the third most prevalent vascular disease worldwide, seriously threatening human health. Baicalin, a flavonoid isolated from the roots of , has been identified to play a crucial role in various vascular diseases. The study aimed to explore the efficacy and underlying mechanisms of baicalin in DVT.
Endothelial progenitor cells (EPCs) were differentiated from peripheral blood mononuclear cells isolated from rat bone marrow. Dil-ac-LDL/FITC-UEA-1 double staining and flow cytometry analysis were conducted for the identification of EPCs. The angiogenesis and migration of EPCs were tested by a tube formation assay and Transwell assay, respectively. DVT rat models were established by stenosis of the inferior vena cava (IVC). After the euthanasia of rats, thrombi in the IVC were collected and weighed, and histological alterations in IVC tissue were measured by H&E staining. The protein levels of SIRT1, p-P65, and p65 in rat IVC tissues were quantified via western blotting.
EPCs used in this study displayed a spindle-like shape and were positive for endothelial cell-specific markers, suggesting the phenotypic characteristics of EPCs. Baicalin enhanced the migratory and angiogenetic abilities of EPCs . For experiments, baicalin reduced thrombus weight and mitigated DVT formation in model rats. Moreover, baicalin activated SIRT but repressed NF-κB signaling in IVC tissues of DVT rats.
Baicalin facilitates migration and angiogenesis of EPCs but impedes thrombus formation via regulation of SIRT1/NF-κB signaling in DVT model rats.
深静脉血栓形成（DVT）是全球第三大常见血管疾病，严重威胁人类健康。黄芩苷是从黄芩根中分离出的一种黄酮类化合物，已被证实在多种血管疾病中发挥关键作用。本研究旨在探讨黄芩苷在DVT中的疗效及潜在机制。
从大鼠骨髓分离的外周血单核细胞中分化出内皮祖细胞（EPCs）。采用Dil-ac-LDL/FITC-UEA-1双重染色和流式细胞术分析鉴定EPCs。分别通过管腔形成试验和Transwell试验检测EPCs的血管生成和迁移能力。通过下腔静脉（IVC）狭窄建立DVT大鼠模型。大鼠安乐死后，收集IVC中的血栓并称重，通过苏木精-伊红（H&E）染色测量IVC组织的组织学改变。通过蛋白质印迹法定量大鼠IVC组织中SIRT1、p-P65和p65的蛋白水平。
本研究中使用的EPCs呈纺锤形，内皮细胞特异性标志物呈阳性，表明具有EPCs的表型特征。黄芩苷增强了EPCs的迁移和血管生成能力。在实验中，黄芩苷降低了模型大鼠的血栓重量并减轻了DVT的形成。此外，黄芩苷激活了DVT大鼠IVC组织中的SIRT，但抑制了NF-κB信号通路。
在DVT模型大鼠中，黄芩苷通过调节SIRT1/NF-κB信号通路促进EPCs的迁移和血管生成，但阻碍血栓形成。
