Department of Medical Biology and Biochemistry, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland.
Department of Cardiology and Clinical Pharmacology, Faculty of Health Sciences, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland.
Clin Interv Aging. 2024 Sep 12;19:1529-1543. doi: 10.2147/CIA.S469624. eCollection 2024.
Glial fibrillary acidic protein (GFAP) and neuroglobin (NGB) are important biomarkers of cerebral hypoxia. For this reason, an attempt was made to assess their concentrations in various time intervals and their impact on the severity of neurological symptoms and functional prognosis of thrombolytic ischemic stroke patients.
The study involved 94 patients reporting to the emergency department of the Collegium Medicum University Hospital in Bydgoszcz within < 4.5 hours of the onset of stroke symptoms. GFAP and neuroglobin levels were measured in plasma at indicated times using a commercial ELISA kit.
Based on the data gathered, statistically significant differences were found between the concentration of biomarkers in stroke patients and the control group. The concentrations of both biomarkers, GFAP and NGB, were elevated in patients after ischemic stroke and the changes in their concentrations in the subsequent stages of stroke may suggest their prognostic value strictly dependent on time. NGB was determined on the 7th day, and mRS - after a year (0.35). GFAP measured after 24 h and on day 7 could be a promising biomarker of functional outcome after one year (cut-off point ≤ 0.231 ng/mL, sensitivity 75.0%, specificity 61.2%, cut off point ≤ 0.235 ng/mL, sensitivity 75.0%, specificity 73.9%, respectively) and the severity of the patient's neurological condition. At GFAP concentrations above 0.25 ng/mL, measured within 24 hours, a sharp increase in mortality was observed in stroke patients. In the case of NGB, at the time of stroke occurrence (14 ng/mL) and after 24 hours (10-60 ng/mL). Differences in the concentrations of these biomarkers have been demonstrated in different stroke subtypes.
NGB and GFAP are important biomarkers of ischemic brain injury and may also participate in predicting neurological outcomes.
胶质纤维酸性蛋白（GFAP）和神经球蛋白（NGB）是脑缺氧的重要生物标志物。因此，尝试评估它们在不同时间间隔的浓度及其对溶栓缺血性脑卒中患者神经症状严重程度和功能预后的影响。
本研究纳入了 94 名在脑卒中症状发作后 4.5 小时内到比得哥什科美纽斯大学医院急诊就诊的患者。在指定时间使用商业 ELISA 试剂盒测量血浆中 GFAP 和 NGB 的浓度。
根据收集的数据，脑卒中患者和对照组的生物标志物浓度存在统计学显著差异。缺血性脑卒中后两种生物标志物 GFAP 和 NGB 的浓度升高，其在脑卒中后各阶段的浓度变化可能提示其预后价值严格依赖于时间。NGB 在第 7 天确定，mRS 在 1 年后为 0.35。在 24 小时和第 7 天测量的 GFAP 可能是 1 年后功能预后的有前途的生物标志物（截断值≤0.231ng/mL，灵敏度 75.0%，特异性 61.2%，截断值≤0.235ng/mL，灵敏度 75.0%，特异性 73.9%）和患者神经状况的严重程度。在 24 小时内测量的 GFAP 浓度超过 0.25ng/mL 时，脑卒中患者的死亡率明显增加。对于 NGB，在脑卒中发生时（14ng/mL）和 24 小时后（10-60ng/mL）。这些生物标志物的浓度在不同的脑卒中亚型中存在差异。
NGB 和 GFAP 是缺血性脑损伤的重要生物标志物，也可能参与预测神经学结局。
