Soraya Cut, Batubara Fitri Yunita, Nasroen Saskia L, Jakfar Subhaini, Gani Basri A
Department of Dentistry Conservative, Dentistry Faculty, University of Syiah Kuala, Darussalam, Banda Aceh, Aceh, Indonesia.
Department of Conservative Dentistry, Dentistry Faculty, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.
J Adv Pharm Technol Res. 2024 Jul-Sep;15(3):200-207. doi: 10.4103/JAPTR.JAPTR_442_23. Epub 2024 Jul 22.
The principal etiological agent responsible for dental caries is ). The () possesses antioxidant and antibacterial properties that function through the response to oxidative stress, which affects bacterial cell metabolism. This research examined impact on growth, toxicity, glucan-binding protein (GBP) expression, and nucleic acid structure. Methods included spectrophotometry for growth analysis, enzyme-linked immunosorbent assay for GBP quantification, the (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) MTT assay for cytotoxicity, Fourier transform infrared for nucleic acid changes, and docking simulation for ligand-receptor affinity. Results showed that significantly inhibited growth at all concentrations over 24 and 48 h (optical density <0.1), comparable to <300 CFU/mL. At 72 h, 6.25% and 3.125% concentrations were most effective, with chlorhexidine also showing stability at these times. A 3.125% concentration of notably reduced GBP production to below 15% and caused cell toxicity. Furthermore, 25% and 3.125% concentrations significantly altered nucleic acids, and showed high binding affinity to the GBP gene receptor. Thus, can inhibit growth and GBP production, cause nucleic acid deformation, and strongly bind to the GBP receptor, highlighting its potential in dental caries prevention.
导致龋齿的主要病原体是（）。（）具有抗氧化和抗菌特性，通过对氧化应激的反应发挥作用，而氧化应激会影响细菌细胞代谢。本研究考察了（）对（）生长、毒性、葡聚糖结合蛋白（GBP）表达和核酸结构的影响。方法包括用分光光度法进行生长分析、用酶联免疫吸附测定法对GBP进行定量、用（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐）MTT法检测细胞毒性、用傅里叶变换红外光谱法检测核酸变化以及用对接模拟法检测配体-受体亲和力。结果表明，在24小时和48小时内，所有浓度的（）均显著抑制（）生长（光密度<0.1），与<300 CFU/mL相当。在72小时时，6.25%和3.125%的浓度最有效，洗必泰在这些时间也表现出稳定性。3.125%浓度的（）显著降低GBP产量至15%以下并导致细胞毒性。此外，25%和3.125%的浓度显著改变（）核酸，并且（）与GBP基因受体表现出高结合亲和力。因此，（）可抑制（）生长和GBP产生，导致核酸变形，并与GBP受体强烈结合，突出了其在预防龋齿方面的潜力。
