Ku Ji-Yeong, Jung Youngwoo, Kim Youngjun, Choi Kyoung-Seong, Park Jinho
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk University, Iksan, Republic of Korea.
Department of Animal Science and Biotechnology, College of Ecology and Environmental Science, Kyungpook National University, Sangju, Republic of Korea.
Vector Borne Zoonotic Dis. 2025 Feb;25(2):92-99. doi: 10.1089/vbz.2024.0060. Epub 2024 Sep 18.
Infection with tick-borne pathogens (TBPs) causes anemia, jaundice, and growth retardation in cattle. Many studies have conducted antigen (Ag) tests for major TBPs, such as spp. and spp., in Korean indigenous cattle (KIC); however, few studies have analyzed antibodies (Ab) against these pathogens. This study simultaneously tested 15 housed cattle raised indoor for over a year and 67 grazing cattle for spp. and spp. Ag using polymerase chain reaction analysis and Ab using enzyme-linked immunosorbent assay. The was detected in 3 housed cattle (20.0%) and 30 grazing cattle (44.8%), whereas the was detected in 3 housed cattle (20.0%) and 54 grazing cattle (80.6%). The positivity rates for spp. Ab did not differ significantly between housed and grazing cattle (4 out of 15 [93.3%] and 55 out of 67 [80.0%], respectively). The positivity rates for spp. Ab were higher in grazing cattle than in housed cattle (21 of 67 [31.3%] and 0 out of 15 [0.0%], respectively) ( < 0.01). No difference was observed between the frequency of grazing and the abundance of Ab against either pathogen. Meanwhile, as a result of comparing the detection of pathogen Ag and Ab, spp. showed the highest proportions of 73.3% and 47.8% in Ag (-)/Ab (+) for housed cattle and grazing cattle, respectively. spp. showed the highest rates of 80.0% in Ag (-)/Ab (-) for housed cattle and 52.2% in Ag (+)/Ab (-) for grazing cattle. This is the first study to determine the impact of antibody abundance against spp. and spp. on antigen prevalence in KIC.
蜱传病原体（TBPs）感染会导致牛出现贫血、黄疸和生长发育迟缓。许多研究已对韩国本土牛（KIC）体内的主要蜱传病原体（如 spp. 和 spp.）进行了抗原（Ag）检测；然而，针对这些病原体的抗体（Ab）分析研究较少。本研究采用聚合酶链反应分析同时检测了15头在室内饲养一年以上的圈养牛和67头放牧牛体内的 spp. 和 spp. 抗原，并采用酶联免疫吸附测定法检测抗体。在3头圈养牛（20.0%）和30头放牧牛（44.8%）中检测到了 ，而在3头圈养牛（20.0%）和54头放牧牛（80.6%）中检测到了 。圈养牛和放牧牛中 spp. 抗体的阳性率无显著差异（分别为15头中的4头[93.3%]和67头中的55头[80.0%]）。放牧牛中 spp. 抗体的阳性率高于圈养牛（分别为67头中的21头[31.3%]和15头中的0头[0.0%]）（P<0.01）。放牧频率与针对这两种病原体的抗体丰度之间未观察到差异。同时，作为比较病原体抗原和抗体检测结果的结果， spp. 在圈养牛和放牧牛的Ag（-）/Ab（+）中分别显示出最高比例，为73.3%和47.8%。 spp. 在圈养牛的Ag（-）/Ab（-）中显示出最高比例，为80.0%，在放牧牛的Ag（+）/Ab（-）中显示出最高比例，为52.2%。这是第一项确定韩国本土牛中针对 spp. 和 spp. 的抗体丰度对抗原流行率影响的研究。
