The interactive effects of gestational obesity and maternal high- and normal-protein diets on food intake, body weight, composition, and glucose metabolism in male offspring of obese Wistar rats.

More than two-thirds of women during childbearing years (20-39 years old) are overweight or obese in the United States, with protein intake among 20-49-year-old women being 1.6 times higher than recommended (75.4 g/day versus 46 g/day) that can be considered as a relatively high-protein diet (HPD). Both gestational obesity and HPDs during gestation adversely affect offspring health. This study investigates the impact of HPDs fed during gestation and lactation on obese mothers and their offspring in Wistar rats. Dams randomized to either a normal-protein diet (NPD) or HPD ( = 12/group). Pups from each maternal group were weaned to either NPD or HPD for 17 weeks ( = 12/group). No effect of maternal or weaning diet on food intake, body weight, or body fat/weight ratio was observed. However, NPD dams exhibited higher glucose area under the curve compared with HPD dams ( < 0.03). At weaning, offspring born to NPD dams showed higher fasting plasma glucose ( < 0.03) and insulin/glucose ratio ( = 0.05) than those born to HPD dams. The Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) index was higher in offspring born to NPD dams ( < 0.04) and weaned to NPD ( < 0.05) at week 17. These findings underscore the role of high-protein maternal and weaning diets in pregnancy outcomes for obese mothers, particularly in glucose homeostasis, although gestational obesity may overshadow other parameters. Further research is needed to fully understand the impact on both maternal and offspring health and their underlying mechanisms in this context.