Frontiers Science Center for Animal Breeding and Sustainable Production, College of Animal Sciences and Technology and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
College of Animal Science and Technology, Shihezi University, Shihezi, China.
FASEB J. 2024 Sep 30;38(18):e70052. doi: 10.1096/fj.202400574R.
Oogenesis involves two phases: initial volumetric growth driven by nutrient accumulation and subsequent nuclear maturation. While melatonin (MLT) has been employed as a supplement to enhance the quality of fully grown oocytes during nuclear maturation phase, its impact on oocyte growth remains poorly studied. Here, we provide in vivo evidence demonstrating that follicle-stimulating hormone increases MLT content in ovary. Administration of MLT improves oocyte growth and quality in mice and goats by enhancing nutrient reserves and mitochondrial function. Conversely, MLT-deficient mice have smaller oocytes and dysfunctional mitochondria. Exploring the clinical implications of MLT in promoting oocyte growth, we observe that a brief 2-day MLT treatment enhances oocyte quality and reproductive performance in older mice. These findings highlight the role of MLT in regulating oocyte growth and provide a specific treatment window for optimizing oocyte quality and reproductive performance in female animals.
初始的体积增长阶段由营养物质积累驱动，随后是核成熟阶段。虽然褪黑素（MLT）已被用作补充剂，以在核成熟阶段提高完全成熟卵母细胞的质量，但它对卵母细胞生长的影响仍研究甚少。在这里，我们提供了体内证据，证明卵泡刺激素增加了卵巢中的 MLT 含量。给予 MLT 可通过增加营养储备和线粒体功能来改善小鼠和山羊的卵母细胞生长和质量。相反，MLT 缺乏的小鼠卵母细胞较小，线粒体功能失调。探讨 MLT 在促进卵母细胞生长方面的临床意义时，我们观察到短暂的 2 天 MLT 治疗可提高老年小鼠的卵母细胞质量和生殖性能。这些发现强调了 MLT 在调节卵母细胞生长中的作用，并为优化雌性动物的卵母细胞质量和生殖性能提供了一个特定的治疗窗口。
