Universidade Federal do Pará, Parque de Ciência e Tecnologia, Laboratório de Óleos da Amazônia, Avenida Perimetral da Ciência, Km 01, s/n, Guamá, 66075-750 Belém, PA, Brazil.
Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Pará, Instituto de Ciências Biológicas, Avenida Augusto Côrrea, s/n, Guamá, 66075-110 Belém, PA, Brazil.
An Acad Bras Cienc. 2024 Sep 13;96(suppl 1):e20230273. doi: 10.1590/0001-3765202420230273. eCollection 2024.
The high performance of biomass and metabolite biosynthesis by photosynthetic microorganisms is directly influenced by the cultivation system employed. Photobioreactors (PBRs) stand out as controlled and fundamental systems for increasing the production of biocompounds. However, the high costs associated with these systems hinder their viability. Thus, a more practical and economical approach is necessary. Accordingly, this study aimed to design and evaluate low-cost flat-panel photobioreactors on a laboratory scale for the cultivation of photosynthetic microorganisms, using economical materials and instruments. Additionally, internal optimization of the low-cost system was aimed to maximize growth and biomass production. The PBRs were designed and built with uniform dimensions, employing 4 mm translucent glass and agitation through compressors. The internally optimized system (PBR-OII) was equipped with perforated acrylic plates used as static mixers. To evaluate the performance of the low-cost PBR-OII, a comparison was made with the control photobioreactor (PBR-CI), of the same geometry but without internal optimization, using a culture of Synechocystis sp. CACIAM 05 culture. The results showed that the PBR-OII achieved maximum biomass yield and productivity of 6.82 mg/mL and 250 mg/L/day, respectively, values superior to the PBR-CI (1.87 mg/mL and 62 mg/L/day). Additionally, the chlorophyll concentration in the PBR-OII system was 28.89 ± 3.44 µg/mL, while in the control system, the maximum reached was 23.12 ± 1.85 µg/mL. Therefore, low-cost photobioreactors have demonstrated to be an essential tool for significantly increasing biomass production, supporting research, and reducing costs associated with the process, enabling their implementation on a laboratory scale.
光合微生物的生物量和代谢产物生物合成的高性能直接受到所采用的培养系统的影响。光生物反应器（PBR）是提高生物化合物产量的可控基础系统。然而，这些系统相关的高成本阻碍了它们的可行性。因此，需要一种更实用和经济的方法。因此，本研究旨在设计和评估用于光合微生物培养的低成本平板光生物反应器，使用经济的材料和仪器。此外，旨在通过内部优化低成本系统以最大化生长和生物量生产。PBR 采用统一尺寸设计和制造，使用 4 毫米半透明玻璃和压缩机搅拌。内部优化系统（PBR-OII）配备了用作静态混合器的穿孔亚克力板。为了评估低成本 PBR-OII 的性能，将其与相同几何形状但没有内部优化的对照光生物反应器（PBR-CI）进行了比较，使用 Synechocystis sp. CACIAM 05 培养物。结果表明，PBR-OII 实现了 6.82mg/mL 的最大生物量产率和 250mg/L/天的生产力，优于 PBR-CI（1.87mg/mL 和 62mg/L/天）。此外，PBR-OII 系统中的叶绿素浓度为 28.89±3.44μg/mL，而对照系统中最大浓度为 23.12±1.85μg/mL。因此，低成本光生物反应器已被证明是显著增加生物量生产、支持研究和降低相关过程成本的重要工具，使其能够在实验室规模上实施。
