HOXA9 促进肝癌的增殖、转移，并抑制其细胞凋亡。
HOXA9 promotes proliferation, metastasis and prevents apoptosis in hepatocellular carcinoma.
机构信息
Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing , 210008, Jiangsu Province, China.
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei , 230022, Anhui Province, China.
出版信息
J Cancer Res Clin Oncol. 2024 Sep 18;150(9):422. doi: 10.1007/s00432-024-05950-9.
PURPOSE
This research aimed to evaluate the expression level of Homeobox A9 (HOXA9) and its role in tumorigenesis of hepatocellular carcinoma (HCC).
METHODS
Bioinformatic analysis, qPCR and Western blot analysis of clinical samples were employed to evaluate mRNA and protein levels of HOXA9 in HCC patients and cell lines. In vitro cell proliferation, migration and invasion, cloning formation, xenograft tumor model, wound healing and apoptosis assays, RNA sequencing analysis of RPL38-silenced HCC-LM3 cells and qPCR, Western blot analysis were performed for validation. Analysis of HOXA9-related genes were conducted to identify their relationships between HOXA9.
RESULTS
HOXA9 is dramatically upregulated in HCC. Upregulation of HOXA9 in HCC predicts poor survival of patients. Besides, HOXA9 promotes proliferation, metastasis and prevents apoptosis in HCC in vitro. In addition, HOXA9 controlled by Ribosomal protein RPL38 is upregulated in HCC. Bioinformatic analysis also indicated that HOXA9 is involved in the regulation of DNA methylation and immune infiltration in HCC.
CONCLUSION
HOXA9 is dramatically upregulated in hepatocellular carcinoma and predicts poor prognosis. Besides, HOXA9 promoted proliferation and metastasis and prevented apoptosis in vitro, which is regulated by Ribosomal protein RPL38 in HCC.
目的
本研究旨在评估同源盒 A9（HOXA9）的表达水平及其在肝细胞癌（HCC）发生中的作用。
方法
通过生物信息学分析、qPCR 和临床样本的 Western blot 分析，评估了 HCC 患者和细胞系中 HOXA9 的 mRNA 和蛋白水平。进行了体外细胞增殖、迁移和侵袭、克隆形成、异种移植肿瘤模型、划痕愈合和细胞凋亡实验、沉默 RPL38 的 HCC-LM3 细胞的 RNA 测序分析以及 qPCR、Western blot 分析进行验证。对 HOXA9 相关基因进行了分析，以确定它们与 HOXA9 之间的关系。
结果
HOXA9 在 HCC 中显著上调。HCC 中 HOXA9 的上调预示着患者预后不良。此外，HOXA9 在体外促进 HCC 的增殖、转移并阻止细胞凋亡。此外，HOXA9 受核糖体蛋白 RPL38 调控，在 HCC 中上调。生物信息学分析还表明，HOXA9 参与了 HCC 中 DNA 甲基化和免疫浸润的调节。
结论
HOXA9 在肝细胞癌中显著上调，预示着预后不良。此外，HOXA9 在体外促进了增殖、转移并阻止了细胞凋亡，这是由 HCC 中的核糖体蛋白 RPL38 调节的。
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