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玉米麦芽中提取的酶对木薯粉(Manihot esculenta Crantz)消化率的影响

Effect of the Digestibility of Cassava Flour (Manihot esculenta Crantz) by Enzymes Extracted from Corn Malt.

作者信息

Amisi Arthur K, Chimanuka Erick A, Kasereka Guelor L, Kizungu Roger V, Bwanganga Jean-Claude T

机构信息

Fermentation and Distillation Laboratory, Department of Chemistry and Agricultural Industries, Faculty of Agricultural Sciences and Environment, University of Kinshasa, PO Box. 14071, Kinshasa 1, Democratic Republic of Congo.

Research-Innovations Laboratory in Agri-Food Industries (LRIIAA), Bandundu-ville, Democratic Republic of Congo.

出版信息

Appl Biochem Biotechnol. 2025 Feb;197(2):837-846. doi: 10.1007/s12010-024-05044-9. Epub 2024 Sep 18.

Abstract

The aim of this study is to determine the effect of the digestibility of cassava starch by the enzymes extracted from corn malt, which will constitute one of the answers to the problem of integrating local products into the process in a modern brewery. Cassava starch solutions of different concentrations (E0: 0 g/L; E1: 1 g/L; E2: 1.1 g/L; E3: 1.2 g/L; E4: 1.3 g/L; E5: 1.4 g/L and E6: 1.5 g/L) were prepared and subjected to two treatments (gelatinized and non-gelatinized) and 5 mL of each were placed in a test tube. Three millilitres (3 mL) of the solution containing amylases extracted from malt corn was then added to each of the test tubes containing the cassava flour solutions. All the treatments were subjected to three temperature stages (50 °C for 15 min, 90 °C for 20 min, and 100 °C for 75 min). Twenty-eight (28) objects (two duplicates) were experimented in a complete factorial design (2 treatments × 2 temperature levels). The results obtained showed that gelatinization had no effect, which could be due to the high optimum temperatures of corn enzyme activity. The concentrations also did not have significant differences which shows that these concentrations can well be used on an industrial scale to digest cassava starch by corn malt enzymes.

摘要

本研究的目的是确定从玉米麦芽中提取的酶对木薯淀粉消化率的影响,这将是解决现代啤酒厂将本地产品融入生产过程这一问题的答案之一。制备了不同浓度的木薯淀粉溶液(E0:0 g/L;E1:1 g/L;E2:1.1 g/L;E3:1.2 g/L;E4:1.3 g/L;E5:1.4 g/L和E6:1.5 g/L),并进行两种处理(糊化和未糊化),每种处理取5 mL置于试管中。然后向装有木薯淀粉溶液的每个试管中加入3 mL含有从麦芽玉米中提取的淀粉酶的溶液。所有处理均经历三个温度阶段(50℃保持15分钟、90℃保持20分钟、100℃保持75分钟)。采用完全析因设计(2种处理×2个温度水平)对28个样本(两个重复)进行实验。所得结果表明糊化没有影响,这可能是由于玉米酶活性的最适温度较高。浓度之间也没有显著差异,这表明这些浓度可很好地用于工业规模,通过玉米麦芽酶消化木薯淀粉。

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