Beani J C, Gautron R, Amblard P, Bastrenta F, Harrouch L, Jardon P, Reymond J L
Photodermatol. 1985 Apr;2(2):101-6.
An original method is described for detecting the photosensitizing ability of a compound. The principle of this method is based on the analysis of variations in the consumption of oxygen by Bacillus subtilis (measured with Warburg's apparatus or an oxygenometric cell), induced by irradiation of the test compound added to the bacterial culture medium. This methodology was applied to 7 substances: 5 known photosensitizers (8-MOP, chlorpromazine, 5-fluorouracil, Vitamin A acid and benzoyl peroxide) and 2 products without any photoactive properties (aspirin and erythromycin). The comparison of results obtained with the method of photo-patch tests and the analysis of the photophysical properties of the compounds confirm the reliability, reproducibility and the quantitative nature of this method.
描述了一种用于检测化合物光敏能力的原始方法。该方法的原理基于对添加到细菌培养基中的受试化合物进行辐照后，枯草芽孢杆菌耗氧量变化的分析（用瓦氏呼吸仪或氧测定仪测量）。该方法应用于7种物质：5种已知的光敏剂（8-甲氧基补骨脂素、氯丙嗪、5-氟尿嘧啶、维甲酸和过氧化苯甲酰）和2种无任何光活性的产品（阿司匹林和红霉素）。用光斑试验方法得到的结果与化合物光物理性质分析结果的比较证实了该方法的可靠性、可重复性和定量性质。
