Department of Orthopedics, 521 Hospitai of Norinco Group.
Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University.
Neuroreport. 2024 Nov 6;35(16):1019-1029. doi: 10.1097/WNR.0000000000002091. Epub 2024 Sep 12.
This study aims to investigate the effect of adipose-derived stem cells (ADSCs) transplantation on progranulin (PGRN) expression and functional recovery in rats with spinal cord injury (SCI). ADSCs were isolated from the inguinal adipose tissue of rats. A SCI model was created, and ADSCs were injected into the injured area. Various techniques were used to assess the effects of ADSCs transplantation, including hematoxylin-eosin staining, Masson staining, immunofluorescence staining, electron microscopy, MRI, and motor function assessment. The potential mechanisms of ADSC transplantation were investigated using gene expression analysis and protein analysis. Finally, the safety of this therapy was evaluated through hematoxylin-eosin staining and indicators of liver and kidney damage in serum. PGRN expression increased in the injured spinal cord, and ADSCs transplantation further enhanced PGRN levels. The group that received ADSCs transplantation showed reduced inflammation, decreased scar formation, increased nerve regeneration, and faster recovery of bladder function. Importantly, motor function significantly improved in the ADSC transplantation group. ADSCs transplantation enhances functional regeneration in SCI by upregulating PGRN expression, reducing inflammation and scar formation, and promoting nerve regeneration and myelin repair. These findings suggest that ADSC transplantation is a potential therapy for SCI.
本研究旨在探讨脂肪来源干细胞（ADSCs）移植对脊髓损伤（SCI）大鼠颗粒体蛋白前体（PGRN）表达和功能恢复的影响。从大鼠腹股沟脂肪组织中分离 ADSCs。建立 SCI 模型，并将 ADSCs 注射到损伤区域。使用各种技术评估 ADSCs 移植的效果，包括苏木精-伊红染色、Masson 染色、免疫荧光染色、电子显微镜、MRI 和运动功能评估。通过基因表达分析和蛋白质分析研究 ADSC 移植的潜在机制。最后，通过苏木精-伊红染色和血清中肝肾功能损伤指标评估该疗法的安全性。PGRN 在损伤的脊髓中表达增加，ADSCs 移植进一步增强了 PGRN 水平。接受 ADSCs 移植的组表现出炎症减轻、疤痕形成减少、神经再生增加和膀胱功能恢复更快。重要的是，运动功能在 ADSC 移植组显著改善。ADSCs 移植通过上调 PGRN 表达、减少炎症和疤痕形成以及促进神经再生和髓鞘修复来增强 SCI 中的功能再生。这些发现表明 ADSC 移植是 SCI 的一种潜在治疗方法。
