The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, China.
Turk Neurosurg. 2024;34(5):888-897. doi: 10.5137/1019-5149.JTN.45113-23.2.
To assess the effect of intravenously injected superparamagnetic iron oxide nanoparticle (SPION)-labeled adipose-derived stem cells (ADSCs) under an external magnetic field on the efficacy of ADSC transplantation in rats with spinal cord injury (SCI).
ADSCs were isolated from rats, labeled with SPIONs, and divided into magnetic and non-magnetic groups. A rat model of SCI was established, and SCI rats were randomly divided into magnetic, non-magnetic, and control groups, with ten rats in each group. Rats in the magnetic and non-magnetic groups were injected with SPION-labeled ADSCs via the tail vein. A 300-mT neodymium iron boron magnet was placed externally at the SCI site of the rats in the magnetic group. One and two weeks after successful modeling, SCI rats were scored for the degree of SCI followed by histopathology of the spinal cord, number of ADSCs at the SCI site, and growth-associated protein-43 (GAP-43) expression were determined in the spinal cord tissues.
One and two weeks after modeling, the Basso-beattie bresnahan (BBB) scores were the highest in the magnetic group, followed by the non-magnetic group, and the lowest in the control group. HE staining showed that the histopathological manifestations of the spinal cord in the magnetic group were somewhat improved compared to those in the non-magnetic and control groups. Two weeks after modeling, Prussian blue staining revealed that the number of ADSCs was significantly higher in the spinal cord tissue of the magnetic group than in that of the non-magnetic group. One and two weeks after modeling, western blotting revealed that the magnetic group exhibited the highest GAP-43 expression.
An external magnetic field applied at the SCI site in rats exerted a directional effect on SPION-labeled ADSCs, directing their migration and improving the efficacy of stem cell-targeted therapies for SCI.
评估在外部磁场下静脉注射超顺磁氧化铁纳米颗粒（SPION）标记的脂肪来源干细胞（ADSCs）对脊髓损伤（SCI）大鼠 ADSC 移植疗效的影响。
从大鼠中分离 ADSC，用 SPION 标记，并分为磁组和非磁组。建立大鼠 SCI 模型，将 SCI 大鼠随机分为磁组、非磁组和对照组，每组 10 只。磁组和非磁组大鼠通过尾静脉注射 SPION 标记的 ADSC。将 300mT 钕铁硼磁铁置于磁组大鼠 SCI 部位的外部。在成功建模后 1 周和 2 周，对 SCI 大鼠进行 SCI 程度评分，然后对脊髓组织中的 ADSC 数量和生长相关蛋白-43（GAP-43）表达进行组织病理学检查。
建模后 1 周和 2 周，磁组的 Basso-beattie bresnahan（BBB）评分最高，其次是非磁组，对照组最低。HE 染色显示，与非磁组和对照组相比，磁组脊髓的组织病理学表现有所改善。建模后 2 周，普鲁士蓝染色显示磁组脊髓组织中 ADSC 的数量明显高于非磁组。建模后 1 周和 2 周，Western blot 显示磁组 GAP-43 表达最高。
在大鼠 SCI 部位施加外部磁场对 SPION 标记的 ADSC 产生了定向作用，引导其迁移，提高了 SCI 干细胞靶向治疗的疗效。
