Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA 30602, USA.
J Chromatogr A. 2024 Nov 8;1736:465378. doi: 10.1016/j.chroma.2024.465378. Epub 2024 Sep 14.
There has been a significant increase in the use of hydrophilic interaction liquid chromatography (HILIC) to separate oligonucleotides. This rise in the use of HILIC has correlated to the increasing success of oligonucleotides as therapeutic treatments and reagents in biomedical research. As more scientists need to routinely analyze oligonucleotides in addition to small molecules, peptides, and proteins using the same analytical instruments, it becomes difficult to use traditional types of analyses such as ion-pair reversed-phase chromatography. This increased use has led to new approaches that have improved the utility of HILIC to the point where it has become a legitimate alternative approach to ion-pair reversed-phase chromatography. This review highlights recent advances in HILIC separations of oligonucleotides with a focus on the underlying mechanisms of action. While HILIC has made significant gains in performance, there still remain challenges, which if properly addressed will continue to propel this approach forward.
亲水作用色谱（HILIC）在分离寡核苷酸中的应用显著增加。HILIC 的这种使用增加与寡核苷酸作为治疗药物和生物医学研究中的试剂的日益成功相关。随着越来越多的科学家需要在使用相同的分析仪器分析小分子、肽和蛋白质的同时常规分析寡核苷酸，使用传统类型的分析方法（如离子对反相色谱法）变得困难。这种增加的使用导致了新的方法的出现，这些方法提高了 HILIC 的实用性，以至于它已经成为离子对反相色谱法的一种可行替代方法。本文综述了 HILIC 分离寡核苷酸的最新进展，重点介绍了作用机制。虽然 HILIC 在性能方面取得了重大进展，但仍然存在挑战，如果得到妥善解决，将继续推动这一方法的发展。
