环状 RNA circRNA_100349 通过调节 IGF2 表达作为 miR-218-5p 的海绵抑制胃癌细胞增殖。

Circular RNA circRNA_100349 functions as a miR-218-5p sponge for suppressing the cell proliferation of gastric cancer via regulation of IGF2 expression.

机构信息

Blood Transfusion Department, The First Hospital of Putian City, Putian, Fujian, China.

Internal Medicine-Oncology, Guangzhou Royal Cancer Hospital, Guangzhou, Guangdong, China.

出版信息

Clinics (Sao Paulo). 2024 Sep 17;79:100492. doi: 10.1016/j.clinsp.2024.100492. eCollection 2024.

DOI:10.1016/j.clinsp.2024.100492

PMID:39293372

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11422554/

Abstract

BACKGROUND

Circular RNAs (circRNAs) hold critical importance due to their notable function in developing Gastric Cancer (GC), which is a malignancy with the third most frequent occurrence worldwide. The aim of this study was to see if circRNA_0044516 would control GC cell proliferation and establish more effective therapeutic strategies.

METHODS

In GC tissues or cells, quantitative Real‑Time Polymerase Chain Reaction (qRT-PCR) was employed for the detection of the expression of circRNA_100349, Insulin-like Growth Factor II (IGF2), and miR-218-5p. CCK-8 assays were employed to gauge the proliferation of cells. A luciferase reporter was employed to establish the relationship of circRNA_100349 or IGF2 with miR-218-5p.

RESULTS

CircRNA_100349 was observed to undergo upregulation in GC cell lines along with tissues. GC cell proliferation was prevented by downregulating circRNA_100349. MiR-149 was targeted by CircRNA_100349, and its downregulation increased the amount of miR-218-5p in GC cells. Simultaneously silencing circRNA_100349 decreased IGF2 expression via miR-218-5p, and thus suppressed GC cell proliferation. Furthermore, in nude mice, circRNA_100349 knockdown prevented the tumor development of GC cells.

CONCLUSIONS

The findings furnished evidence of the critical involvement of circRNA_100349 in GC and that its downregulation impedes GC cell proliferation via the miR-218-5p/IGF2 axis.

摘要

背景

环状 RNA（circRNAs）因其在胃癌（GC）发展中的重要作用而备受关注，GC 是全球第三大常见恶性肿瘤。本研究旨在探讨 circRNA_0044516 是否可以控制 GC 细胞增殖并建立更有效的治疗策略。

方法

在 GC 组织或细胞中，采用实时定量聚合酶链反应（qRT-PCR）检测 circRNA_100349、胰岛素样生长因子 II（IGF2）和 miR-218-5p 的表达。采用 CCK-8 法检测细胞增殖。采用荧光素酶报告基因检测 circRNA_100349 或 IGF2 与 miR-218-5p 的关系。

结果

在 GC 细胞系和组织中观察到 circRNA_100349 上调。下调 circRNA_100349 可抑制 GC 细胞增殖。CircRNA_100349 靶向 miR-149，其下调可增加 GC 细胞中 miR-218-5p 的含量。同时沉默 circRNA_100349 通过 miR-218-5p 降低 IGF2 表达，从而抑制 GC 细胞增殖。此外，在裸鼠中，circRNA_100349 敲低可阻止 GC 细胞的肿瘤发展。