Rosenblatt H M, Ulrich R G, Shearer W T
Res Commun Chem Pathol Pharmacol. 1985 Jul;49(1):35-45.
Monoclonal anti-actin antibody which is known to stimulate DNA synthesis and cell growth in a murine transformed cell line, L cell, was examined for its ability to modulate the expression of surface antigen on the cell membrane. There was a time dependent increase in the number of cell surface antigen molecules on L cells stimulated by monoclonal anti-actin antibody as measured by indirect immunofluorescence and flow cytometry. For L cells incubated for 12, 24, 48, 72, and 96 hours with monoclonal anti-actin antibody versus a myeloma control supernatant, the percentage of cells exhibiting high intensity immunofluorescence was 29 vs. 13, 13 vs. 10, 34 vs. 1, 52 vs. 11, and 35 vs. 18, respectively. We conclude that monoclonal anti-actin antibody modulates the time dependent surface expression of actin on L cells. It is likely that this modulated expression of surface actin-like molecules plays an important role in the transmission of stimulatory signals to the cells which result in enhanced cellular metabolism and proliferation.
已知单克隆抗肌动蛋白抗体可刺激小鼠转化细胞系L细胞中的DNA合成和细胞生长，研究了其调节细胞膜表面抗原表达的能力。通过间接免疫荧光和流式细胞术测量，发现单克隆抗肌动蛋白抗体刺激的L细胞表面抗原分子数量呈时间依赖性增加。与骨髓瘤对照上清液相比，用单克隆抗肌动蛋白抗体孵育12、24、48、72和96小时的L细胞，显示高强度免疫荧光的细胞百分比分别为29%对13%、13%对10%、34%对1%、52%对11%和35%对18%。我们得出结论，单克隆抗肌动蛋白抗体调节L细胞上肌动蛋白的时间依赖性表面表达。表面肌动蛋白样分子的这种调节表达可能在向细胞传递刺激信号中起重要作用，从而导致细胞代谢和增殖增强。
