College of Life Science, Qingdao University, Qingdao, Shandong, 266071, China.
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Anal Biochem. 2025 Jan;696:115672. doi: 10.1016/j.ab.2024.115672. Epub 2024 Sep 16.
The integration of fiber optics and plasmonic sensors is promising to improve the practical usability over conventional bulky sensors and systems. To achieve high sensitivity, it typically requires fabrication of well-defined plasmonic nanostructures on optical fibers, which greatly increases the cost and complexity of the sensors. Here, we present a fiber-optic sensor system by using chemical absorption of gold nanoparticles and a replaceable configuration. By functioning gold nanoparticles with aptamers or antibodies, we demonstrate the applications in chemical sensing using two different modes. Measuring shift in resonance wavelength enables the Pb detection with a high linearity and a limit of detection of 0.097 nM, and measuring absorption peak amplitude enables the detection of E. coli in urinary tract infection with a dynamic range between 10 to 10 CFU/mL. The high sensitivity, simple fabrication and disposability of this sensing approach could pave the way for point-of-care testing with fiber-optic plasmonic sensors.
光纤和等离子体传感器的集成有望提高传统大型传感器和系统的实际可用性。为了实现高灵敏度，通常需要在光纤上制造出定义良好的等离子体纳米结构，这大大增加了传感器的成本和复杂性。在这里，我们提出了一种光纤传感器系统，该系统利用金纳米粒子的化学吸收和可替换的结构。通过使金纳米粒子与适体或抗体结合，我们展示了两种不同模式下的化学传感应用。通过测量共振波长的位移，可以实现对 Pb 的高线性检测，检测限为 0.097 nM，通过测量吸收峰幅度，可以实现对尿路感染中大肠杆菌的检测，检测范围在 10 到 10 CFU/mL 之间。这种传感方法具有高灵敏度、简单的制造和可重复使用性，为光纤等离子体传感器的即时检测铺平了道路。
