Department of Biotechnology, Faculty of Science, Selcuk University, 42130, Konya, Turkey.
Department of Molecular Biology and Genetics, Faculty of Science, Necmettin Erbakan University, 42090, Konya, Turkey.
Sci Total Environ. 2024 Dec 1;954:176335. doi: 10.1016/j.scitotenv.2024.176335. Epub 2024 Sep 17.
Organic pollutants, which have become one of the most striking problems of today, raise concerns about the spread of polyaromatic hydrocarbon (PAH) compounds into ecosystems and their toxic effects on living organisms. The purpose of this study was to determine how harmful 3-nitrofluoranthene (3-NF) exposure was to Salvinia natans, a freshwater macrophyte. Furthermore, it clarifies how this aquatic plant, which is frequently used in phytoremediation of water contaminants and wastewater treatments, interacts with PAHs and contributes to the development of bioremediation methods. In S. natans exposed to stress (10 μM (3-NF10), 25 μM (3-NF25), 50 μM (3-NF50), 100 μM (3-NF100), 250 μM (3-NF250), 500 μM (3-NF500), 1000 μM (3-NF1000) 3-nitrofluoranthene), 3-NF accumulation, oxidative stress indicators, photosynthetic efficiency, and antioxidant system activity alterations were investigated for this objective. The findings demonstrated that S. natans could effectively accumulate 3-NF, and at a concentration of 1000 μM, the 3-NF content in the leaves reached approximately 1112 mg/kg. While its adverse effects on growth (RGR) and photosynthesis (F/F) remained mild up to a concentration of 250 μM, the severity of the inhibitions increased at higher concentrations. On the other hand, exposure to 3-NF triggered the antioxidant system in S. natans plants and resulted in an increase of 60 %, 80 %, 47 % and 27 % in superoxide dismutase (SOD) activity in 3-NF10-25-50-100 groups, respectively. Conversely, in comparison to control plants, higher concentrations of 3-NF treatments resulted in insufficient antioxidant activity, increased lipid peroxidation (TBARS concentration), and hydrogen peroxide (HO). In conclusion, S. natans plants tolerated 3-NF accumulation up to 250 μM concentration despite limitations in growth suppression and photosynthetic capacity, proving that S. natans has the potential to be used in phytoextraction studies of 3-NF-polluted waters.
有机污染物已成为当今最引人关注的问题之一，人们担心多环芳烃（PAH）化合物会扩散到生态系统中，并对生物体产生毒性影响。本研究旨在确定 3-硝基芴（3-NF）暴露对水生植物满江红的危害程度，满江红是一种常用于水污染物和废水处理的水生植物。此外，本研究还阐明了这种水生植物如何与 PAHs 相互作用，并为生物修复方法的发展做出贡献。在暴露于应激条件下的满江红（10 μM（3-NF10）、25 μM（3-NF25）、50 μM（3-NF50）、100 μM（3-NF100）、250 μM（3-NF250）、500 μM（3-NF500）、1000 μM（3-NF1000）3-硝基芴）中，研究了 3-NF 积累、氧化应激指标、光合作用效率和抗氧化系统活性的变化。研究结果表明，满江红可以有效地积累 3-NF，在 1000 μM 浓度下，叶片中的 3-NF 含量达到约 1112 mg/kg。在 250 μM 浓度以下，其对生长（RGR）和光合作用（F/F）的不利影响仍然较轻，但在较高浓度下，抑制作用的严重程度增加。另一方面，3-NF 暴露触发了满江红植物的抗氧化系统，导致 SOD 活性分别增加 60%、80%、47%和 27%，在 3-NF10-25-50-100 组中。相比之下，与对照植物相比，较高浓度的 3-NF 处理导致抗氧化活性不足，脂质过氧化（TBARS 浓度）和过氧化氢（HO）增加。总之，尽管生长受到抑制和光合作用能力下降，满江红植物仍能耐受 3-NF 积累至 250 μM 浓度，证明满江红具有在 3-NF 污染水的植物提取研究中应用的潜力。
