Golden S S, Haselkorn R
Science. 1985 Sep 13;229(4718):1104-7. doi: 10.1126/science.3929379.
A psbA gene encoding the target of photosystem II herbicide inhibition, the 32,000-dalton thylakoid membrane protein, has been cloned from a mutant of Anacystis nidulans R2, which is resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea-(diuron). A cloned DNA fragment from within the coding region of this gene transforms wild-type cells to herbicide resistance, proving that mutation within psbA is responsible for that phenotype. The mutation consists of a single nucleotide change that replaces serine at position 264 of the wild-type protein with alanine in that of the diuron-resistant mutant.
编码光系统II除草剂作用靶标的psbA基因，即32,000道尔顿的类囊体膜蛋白，已从集胞藻6803的一个对3-(3,4-二氯苯基)-1,1-二甲基脲（敌草隆）具有抗性的突变体中克隆出来。从该基因编码区内克隆的一个DNA片段可将野生型细胞转化为除草剂抗性细胞，这证明psbA基因内的突变导致了该表型。该突变由单个核苷酸变化组成，在野生型蛋白第264位的丝氨酸在敌草隆抗性突变体中被丙氨酸取代。
