集胞藻6714中对酚类除草剂抗性的突变定位在psbA基因内。
Mutation in phenol-type herbicide resistance maps within the psbA gene in Synechocystis 6714.
作者信息
Ajlani G, Meyer I, Vernotte C, Astier C
机构信息
ER 407, CNRS, Gif-sur-Yvette, France.
出版信息
FEBS Lett. 1989 Mar 27;246(1-2):207-10. doi: 10.1016/0014-5793(89)80284-4.
A Synechocytis 6714 mutant resistant to the phenol-type herbicide ioxynil was isolated and characterized. Sensitivity to DCMU and atrazine was tf measured in whole cells and isolated thylakoids. The mutant presents the same sensitivity to atrazine as the wild type and a slightly increased sensitivity to DCMU. A point mutation has been found at codon 266 in the psbAI coding locus (AAC to ACC) resulting in an amino acid change from asparagine to threonine in the D1 protein.
分离并鉴定了一株对酚类除草剂碘苯腈具有抗性的集胞藻6714突变体。在完整细胞和分离的类囊体中测定了其对二氯苯基二甲脲（DCMU）和莠去津的敏感性。该突变体对莠去津的敏感性与野生型相同，对DCMU的敏感性略有增加。在psbAI编码位点的第266位密码子处发现了一个点突变（AAC突变为ACC），导致D1蛋白中的氨基酸从天冬酰胺变为苏氨酸。
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