Department of Pediatrics, Division of Infectious Diseases, State University of New York Downstate Health Sciences University, Brooklyn, New York, USA
Department of Pediatrics, Division of Infectious Diseases, State University of New York Downstate Health Sciences University, Brooklyn, New York, USA.
Ann Clin Lab Sci. 2024 Jul;54(4):504-509.
() is a gram-negative intracellular bacterium that causes respiratory infections in humans. is responsible for cell activation and production of cytokines that may contribute to inflammatory responses in asthma. Cell-mediated immune responses are important for protective immunity; however, these responses may be impaired in asthma. In this study, we examined cytokine responses (IL-21, IL-12, IL-13) responsible for T helper (Th)1 versus Th2 responses in -stimulated PBMC from subjects with or without asthma. These cytokines could be potential biomarkers in the evaluation of past infection.
Peripheral blood mononuclear cells (PBMC) (1×10/mL) from stable adult asthmatic (N=6) and non-asthmatic subjects (N=6) were infected +/- TW-183 at a multiplicity of infection (MOI)=0.1, using dose responses (1:10, 1:100), and cultured 48 hrs. Cytokine responses (Interleukin (IL)-21, IL-12, IL-13) were measured in supernatants (ELISA).
Cytokine responses (mean differences: unstimulated-stimulated cells) were significant for IL-12 (1:10, 1:100) (=0.0005, 0.0005) but not for IL-21 or IL-13 (Wilcoxon signed-rank test). Cytokine levels were higher in asthmatic subjects for IL-13 (mean differences: non-asthma-asthma) (unstimulated, 1:10, 1:100) (-210±167, -140±113, -89±59, respectively) (=0.05, 0.05, 0.05, respectively) compared with non-asthma. However, IL-21 and IL-12 responses were similar in both groups. When subjects were stratified according to IgG antibody status, no significant differences in cytokine responses were observed.
Differential cytokine patterns in subjects with or without asthma may suggest a mechanism for the development of persistent infection with .
（）是一种革兰氏阴性细胞内细菌，可引起人类呼吸道感染。它负责激活细胞并产生细胞因子，这些细胞因子可能导致哮喘中的炎症反应。细胞介导的免疫反应对于保护性免疫很重要；然而，这些反应在哮喘中可能受损。在这项研究中，我们研究了（）刺激的 PBMC 中负责 Th1 与 Th2 反应的细胞因子（IL-21、IL-12、IL-13）在有或没有哮喘的受试者中的反应。这些细胞因子可能是评估过去感染的潜在生物标志物。
稳定的成年哮喘患者（N=6）和非哮喘患者（N=6）的外周血单核细胞（PBMC）（1×10/mL），以感染复数（MOI）=0.1 感染 +/- TW-183，使用剂量反应（1:10、1:100），并培养 48 小时。细胞因子反应（白细胞介素（IL）-21、IL-12、IL-13）在上清液中（ELISA）进行测量。
细胞因子反应（未刺激-刺激细胞的平均差异）在 IL-12（1:10、1:100）（=0.0005、0.0005）方面有显著差异，但 IL-21 或 IL-13 方面没有（Wilcoxon 符号秩检验）。在哮喘患者中，IL-13 的细胞因子水平更高（非哮喘-哮喘的平均差异）（未刺激，1:10，1:100）（-210±167、-140±113、-89±59）（=0.05、0.05、0.05），与非哮喘患者相比。然而，两组的 IL-21 和 IL-12 反应相似。当根据 IgG 抗体状态对受试者进行分层时，未观察到细胞因子反应的显著差异。
有或没有哮喘的受试者中的细胞因子模式的差异可能表明与持续感染的发展有关。
