A point mutation in VIG1 boosts development and chilling tolerance in rice.

The rice paddy-direct seeding system has been widely adopted due to its low cost and convenience, whereas its application is mainly constrained by low seedling vigor, cold sensitivity, eventually resulting in reduced grain yield. Here, we show vig1a and vig1b, two allelic mutants of OsbZIP01, that both demonstrate greatly enhanced seedling vigor and chilling tolerance but differ in final grain production. The vig1a phenotype can be obtained via simultaneous mutation of the genes OsbZIP01 and OsbZIP18, or by selectively manipulating the basic region of OsbZIP01. Destroying the leucine zipper region of OsbZIP01 in vig1a turns vig1a to be vig1b. Further analysis reveals that OsbZIP01 and OsbZIP18 function cooperatively in diverse crucial biological programs that determine seedling establishment, chilling tolerance, and grain yield through their interactions. These findings provide a strategy toward simultaneously improving seedling vigor, chilling tolerance, and grain yield for rice production.