长期卵巢切除（OVX）大鼠用竞争性促黄体生成素释放激素（LRH）拮抗剂预处理对体外促卵泡生成素（FSH）释放的影响。

Effect of pretreatment of long-term ovariectomized (OVX) rats with a competitive LRH antagonist on FSH release in vitro.

作者信息

van Dieten J A, van Rees G P

出版信息

Acta Endocrinol (Copenh). 1985 Aug;109(4):481-4. doi: 10.1530/acta.0.1090481.

DOI:10.1530/acta.0.1090481

Abstract

The effect of a single sc injection of an LRH antagonist ((Ac-D-p-Cl-Phe1,2,D-Trp3,D-Phe6,D-Ala10)-LRH, Org 30093) into OVX rats on FSH release 24 h later was studied. Plasma FSH was decreased but pituitary FSH content was not changed. Incubation of the pituitary glands during 4 h resulted in a decreased basal release. FSH release induced by a low concentration of LRH (1 ng/ml) was depressed but that of a high concentration (10 000 ng/ml) was augmented in comparison to FSH release induced in control glands. However, pretreatment with the antagonist had no specific effect on FSH release in vitro induced by high K+ or high K+ plus mbcAMP and theophylline, indicating that the changes of pituitary responsiveness to LRH are not caused by those parts of the secretory mechanism which are stimulated by these secretagogues. Moreover, it is concluded that the changes of pituitary LH release induced by administration of an LRH antagonist also concern FSH.

摘要

研究了向去卵巢大鼠单次皮下注射促黄体激素释放激素（LRH）拮抗剂（(Ac-D-p-Cl-Phe1,2,D-Trp3,D-Phe6,D-Ala10)-LRH，Org 30093）对24小时后促卵泡激素（FSH）释放的影响。血浆FSH降低，但垂体FSH含量未改变。垂体在4小时的孵育导致基础释放减少。与对照腺体中诱导的FSH释放相比，低浓度LRH（1 ng/ml）诱导的FSH释放受到抑制，但高浓度（10000 ng/ml）的FSH释放增加。然而，用拮抗剂预处理对高钾或高钾加甲硫氨酸环腺苷酸（mbcAMP）和茶碱在体外诱导的FSH释放没有特异性影响，这表明垂体对LRH反应性的变化不是由这些促分泌剂刺激的分泌机制部分引起的。此外，得出的结论是，给予LRH拮抗剂诱导的垂体促黄体生成素（LH）释放变化也与FSH有关。