Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark.
Department of Clinical Research, University of Southern Denmark, Odense, Denmark.
APMIS. 2024 Nov;132(11):807-813. doi: 10.1111/apm.13469. Epub 2024 Sep 18.
The purpose of this study was to establish a porcine model of urinary tract infection (UTI) with gram-positive uropathogens. Ten female domestic pigs were experimentally inoculated with human UTI isolates of Enterococcus faecalis (n = 3), Staphylococcus saprophyticus (n = 3), or Staphylococcus aureus (n = 4) and followed with regular urine samples. Bladders and kidneys were aseptically removed at termination (5-7 days post infection) and assessed by gross pathology and bacterial enumeration. Enterococcus faecalis (n = 3 of 3) and S. aureus (n = 2 of 4) successfully colonized the pig bladders. Inoculation with S. saprophyticus never resulted in detectable bacteriuria. All infected pigs had cleared the infection spontaneously before termination. Surprisingly, three (of four) pigs inoculated with S. aureus led to spontaneous infection with opportunistic pathogens. Also, one pig colonized with E. faecalis resulted in spontaneous infection with E. coli. In conlusion, the pig supports experimental UTI with E. faecalis for up to 24 h but not prolonged infection. S. aureus and S. saprophyticus fails to cause UTI in pigs and other animals should be considered for studying these pathogens.
本研究旨在建立一种猪模型，用于研究革兰氏阳性尿路病原体引起的尿路感染（UTI）。10 头雌性家猪分别用粪肠球菌（n=3）、腐生葡萄球菌（n=3）或金黄色葡萄球菌（n=4）的人 UTI 分离株进行实验性接种，并定期采集尿液样本。在感染后 5-7 天（终止时），无菌取出膀胱和肾脏，通过大体病理和细菌计数进行评估。粪肠球菌（n=3 of 3）和金黄色葡萄球菌（n=4 of 4）成功定植于猪膀胱。腐生葡萄球菌的接种从未导致可检测的菌尿。所有感染猪在终止前均自发清除了感染。令人惊讶的是，用金黄色葡萄球菌接种的四头猪中有三头（three of four）自发性感染了机会性病原体。此外，一头定植粪肠球菌的猪自发性感染了大肠杆菌。总之，猪支持粪肠球菌引起的长达 24 小时的实验性 UTI，但不支持延长感染。金黄色葡萄球菌和腐生葡萄球菌不能引起猪的 UTI，应该考虑其他动物来研究这些病原体。
