Purification of anti-lactose antibodies from antisera to Neisseria gonorrhoeae and Neisseria meningitidis.

Lactosyl-sepharose 4B columns were used for purification by affinity chromatography of anti-lactose antibodies from rabbit antisera to the gonococcal strains 8551 and VII, and the serogroup B meningococcal strain M982. Anti-lactose antibodies were obtained from all three antisera. SDS-PAGE of lipopolysaccharide and of bacterial cells and immunoblotting with the anti-lactose antibodies showed that the lipopolysaccharide was the only bacterial component with binding sites for the antibodies.