Wang Jun, Hu YuXuan, Xue Yuan, Wang Kai, Mao Dong, Pan Xiao-Yun, Rui YongJun
Suzhou Medical College of Soochow University, Suzhou, Jiangsu, 215000, China.
Department of Orthopedics, Wuxi Ninth People's Hospital Affiliated to Soochow University, Wuxi, Jiangsu, 214000, China.
Heliyon. 2024 Sep 2;10(17):e37231. doi: 10.1016/j.heliyon.2024.e37231. eCollection 2024 Sep 15.
The purpose of this study is to investigate the role of PMMA-induced biofilm in nerve regeneration compared with silicone-induced biofilm involved in the mechanism.
PMMA or silicon rods were placed next to the sciatic nerve to induce a biological membrane which was assayed by PCR, Western blot, immunohistochemistry, immunofluorescence and proteomics. A 10 mm sciatic nerve gaps were repaired with the autologous nerve wrapped in an induced biological membrane. The repair effects were observed through general observation, functional evaluation of nerve regeneration, ultrasound examination, neural electrophysiology, the wet weight ratio of bilateral pretibial muscle and histological evaluation. Cell proliferation and migration of Schwann cells co-cultured with EGF-treated fibroblasts combined with siRNA were investigated.
The results indicated that expression of GDNF, NGF and VEGF along with neovascularization was similar in the silicone and PMMA group and as the highest at 6 weeks after operation. Nerve injury repair mediated by toluidine blue and S100β/NF200 expression, the sensory and motor function evaluation, ultrasound, target organ muscle wet-weight ratio, percentage of collagen fiber, electromyography and histochemical staining were not different between the two groups and better than blank group. EGF-treated fibroblasts promoted proliferation and migration may be Tnc expression dependently.
Our study suggested that PMMA similar to silicon induced biofilm may promote autogenous nerve transplantation to repair nerve defects through EGF/Tnc/FN1 to increase Schwann cells proliferation and migration.
本研究旨在探讨聚甲基丙烯酸甲酯（PMMA）诱导生物膜在神经再生中的作用，并与涉及该机制的硅胶诱导生物膜进行比较。
将PMMA或硅胶棒置于坐骨神经旁以诱导生物膜，通过聚合酶链反应（PCR）、蛋白质免疫印迹法、免疫组织化学、免疫荧光和蛋白质组学对其进行检测。用包裹有诱导生物膜的自体神经修复10毫米的坐骨神经缺损。通过一般观察、神经再生功能评估、超声检查、神经电生理学、双侧胫前肌湿重比和组织学评估来观察修复效果。研究了与经表皮生长因子（EGF）处理的成纤维细胞联合小干扰RNA（siRNA）共培养的雪旺细胞的增殖和迁移情况。
结果表明，硅胶组和PMMA组中胶质细胞源性神经营养因子（GDNF）、神经生长因子（NGF）和血管内皮生长因子（VEGF）的表达以及新生血管形成情况相似，且在术后6周时最高。两组间由甲苯胺蓝及S100β/神经丝蛋白200（NF200）表达介导的神经损伤修复、感觉和运动功能评估、超声、靶器官肌肉湿重比、胶原纤维百分比、肌电图和组织化学染色无差异，且均优于空白组。经EGF处理的成纤维细胞促进增殖和迁移可能依赖于肌腱蛋白C（Tnc）表达。
我们的研究表明，与硅胶诱导生物膜类似，PMMA可能通过表皮生长因子/肌腱蛋白C/纤连蛋白1（EGF/Tnc/FN1）促进自体神经移植修复神经缺损，以增加雪旺细胞的增殖和迁移。
