Arslan Semiha Cömertoğlu, Arslan Feyzullah Necati, Altun Hatice, Taş Dilan, Islah Elif Milhan, Seyithanoğlu Muhammed, Doğaner Adem
Department of Child and Adolescent Psychiatry, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey.
Developmental-Behavioral Pediatrics Division, Department of Pediatrics, Kahramanmaraş Necip Fazil City Hospital, Kahramanmaraş, Turkey.
Int J Dev Neurosci. 2024 Dec;84(8):867-876. doi: 10.1002/jdn.10377. Epub 2024 Sep 19.
This study aimed to ascertain the serum levels of selectins (E, L, P) and platelet-endothelial adhesion molecule-1 (PECAM-1) in preschool children with autism spectrum disorder (ASD) and to establish a comparison with the levels observed in healthy controls.
The study included 34 children aged 2-7 years diagnosed with ASD (ASD group) and 34 randomly selected healthy children matched for age and sex to the ASD group. The children were free of any genetic or physical disease, clinically active infection, or medication use. The sociodemographic data form was completed by all parents. The Childhood Autism Rating Scale (CARS) and the Autism Behavior Checklist (ABC) were administered to the patient group, and the Aberrant Behavior Checklist (AbBC) was completed by the families of all children. Serum selectin (E, L, P) and PECAM-1 levels were measured using enzyme-linked immunosorbent assay (ELISA) kits.
The results showed that the levels of both L-selectin (p = 0.007) and PECAM-1 (p = 0.019) were significantly lower in the ASD group than in the control group. No significant difference was observed between the groups concerning E-selectin and P-selectin levels (p > 0.05). It was observed that P-selectin variables were statistically significant in predicting the presence of ASD (p = 0.019). A remarkable inverse correlation was found between the AbBC irritability subscale score and L-selectin (r = -0.296, p = 0.014) and PECAM-1 (r = -0.276, p = 0. 023); the AbBC Lethargy-Social Withdrawal subscale score and E-Selectin (r = -0.239, p = 0.049), L-Selectin (r = -0.297, p = 0.014) and PECAM-1 (r = -0.264, p = 0.029); L-Selectin levels and the AbBC stereotypic behavior subscale (r = -0.248, p = 0.042). No statistically significant relationship was observed between selectins (E, L, P) and PECAM-1 levels and CARS scale, ABC subscale or total scores and age variables (p > 0.05).
These study results suggest that L-selectin, P-selectin and PECAM-1 may play a role in the pathophysiology of ASD.
本研究旨在确定自闭症谱系障碍（ASD）学龄前儿童血清中选择素（E、L、P）和血小板内皮黏附分子-1（PECAM-1）的水平，并与健康对照者的水平进行比较。
该研究纳入了34名年龄在2至7岁之间被诊断为ASD的儿童（ASD组），以及34名随机选取的年龄和性别与ASD组相匹配的健康儿童。这些儿童没有任何遗传或身体疾病、临床活动性感染或正在使用药物。所有家长均填写了社会人口学数据表格。对患者组进行了儿童自闭症评定量表（CARS）和自闭症行为检查表（ABC）评估，所有儿童的家庭均完成了异常行为检查表（AbBC）。使用酶联免疫吸附测定（ELISA）试剂盒测量血清选择素（E、L、P）和PECAM-1水平。
结果显示，ASD组的L-选择素水平（p = 0.007）和PECAM-1水平（p = 0.019）均显著低于对照组。两组在E-选择素和P-选择素水平方面未观察到显著差异（p > 0.05）。观察到P-选择素变量在预测ASD的存在方面具有统计学意义（p = 0.019）。发现AbBC易激惹分量表得分与L-选择素（r = -0.296，p = 0.014）和PECAM-1（r = -0.276，p = 0.023）之间存在显著的负相关；AbBC嗜睡-社交退缩分量表得分与E-选择素（r = -0.239，p = 0.049）、L-选择素（r = -0.297，p = 0.014）和PECAM-1（r = -0.264，p = 0.029）之间存在显著的负相关；L-选择素水平与AbBC刻板行为分量表之间存在显著的负相关（r = -0.248，p = 0.042）。在选择素（E、L、P）和PECAM-1水平与CARS量表、ABC分量表或总分以及年龄变量之间未观察到统计学上的显著关系（p > 0.05）。
这些研究结果表明，L-选择素、P-选择素和PECAM-1可能在ASD的病理生理学中起作用。
