• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于量子点的肺动脉高压患者肺动脉细胞计数及免疫染色方案

Protocol for quantum dot-based cell counting and immunostaining of pulmonary arterial cells from patients with pulmonary arterial hypertension.

作者信息

Moinuddin Sakib M, Ibrahim Md, Sarkar Tanoy, Hossain Md Shahadat, Rose Melanie, Ahsan Fakhrul

机构信息

Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA.

Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103319. doi: 10.1016/j.xpro.2024.103319. Epub 2024 Sep 18.

DOI:10.1016/j.xpro.2024.103319
PMID:39298320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11426121/
Abstract

Currently, there is no protocol for growing and culturing primary pulmonary arterial cells (PACs) available from the Pulmonary Hypertension Breakthrough Initiative (PHBI). Here, we present a protocol for cultivating and maintaining three major PACs collected from patients with pulmonary arterial hypertension (PAH): endothelial (PAH-ECs), smooth muscle (PAH-SMCs), and adventitial cells (PAH-ADCs). We describe steps for obtaining PACs from PHBI, evaluating the growth of cells labeled with quantum dots (QDs), and staining endothelial cell (EC) markers for immunofluorescence imaging. For complete details on the use and execution of this protocol, please refer to Al-Hilal et al..

摘要

目前，肺动脉高压突破计划（PHBI）没有提供用于培养和培育原代肺动脉细胞（PACs）的方案。在此，我们展示一种用于培养和维持从肺动脉高压（PAH）患者收集的三种主要PACs的方案：内皮细胞（PAH-ECs）、平滑肌细胞（PAH-SMCs）和外膜细胞（PAH-ADCs）。我们描述了从PHBI获取PACs、评估用量子点（QDs）标记的细胞生长情况以及对内皮细胞（EC）标志物进行染色以用于免疫荧光成像的步骤。有关此方案的使用和实施的完整详细信息，请参考Al-Hilal等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/646dfbca80f5/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/c1cc8dbb882f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/d70a1baf5dec/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/5088c13eab4c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/2386e5970c72/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/2c13917330eb/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/839748c23e09/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/86377ef6978f/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/7fcdc70635df/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/00e206913aa3/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/646dfbca80f5/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/c1cc8dbb882f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/d70a1baf5dec/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/5088c13eab4c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/2386e5970c72/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/2c13917330eb/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/839748c23e09/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/86377ef6978f/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/7fcdc70635df/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/00e206913aa3/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/11426121/646dfbca80f5/gr9.jpg

相似文献

1
Protocol for quantum dot-based cell counting and immunostaining of pulmonary arterial cells from patients with pulmonary arterial hypertension.基于量子点的肺动脉高压患者肺动脉细胞计数及免疫染色方案
STAR Protoc. 2024 Dec 20;5(4):103319. doi: 10.1016/j.xpro.2024.103319. Epub 2024 Sep 18.
2
Autophagy contributes to BMP type 2 receptor degradation and development of pulmonary arterial hypertension.自噬促进 BMP 型 2 受体降解和肺动脉高压的发生。
J Pathol. 2019 Nov;249(3):356-367. doi: 10.1002/path.5322. Epub 2019 Aug 27.
3
Blockade of JAK2 protects mice against hypoxia-induced pulmonary arterial hypertension by repressing pulmonary arterial smooth muscle cell proliferation.阻断 JAK2 可通过抑制肺动脉平滑肌细胞增殖来保护小鼠免受低氧诱导的肺动脉高压。
Cell Prolif. 2020 Feb;53(2):e12742. doi: 10.1111/cpr.12742. Epub 2020 Jan 14.
4
Otud6b induces pulmonary arterial hypertension by mediating the Calpain-1/HIF-1α signaling pathway.Otud6b 通过介导钙蛋白酶 1/HIF-1α 信号通路诱导肺动脉高压。
Cell Mol Life Sci. 2024 Jun 15;81(1):258. doi: 10.1007/s00018-024-05291-3.
5
Necrosis-Released HMGB1 (High Mobility Group Box 1) in the Progressive Pulmonary Arterial Hypertension Associated With Male Sex.在与男性相关的进行性肺动脉高压中，坏死释放的高迁移率族蛋白 B1（HMGB1）。
Hypertension. 2020 Dec;76(6):1787-1799. doi: 10.1161/HYPERTENSIONAHA.120.16118. Epub 2020 Oct 5.
6
NOTCH3 and Pulmonary Arterial Hypertension.NOTCH3 与肺动脉高压。
Int J Mol Sci. 2024 Jun 6;25(11):6248. doi: 10.3390/ijms25116248.
7
Panorama of artery endothelial cell dysfunction in pulmonary arterial hypertension.肺动脉高压中动脉内皮细胞功能障碍的全景。
J Mol Cell Cardiol. 2024 Dec;197:61-77. doi: 10.1016/j.yjmcc.2024.10.004. Epub 2024 Oct 20.
8
The novel roles of YULINK in the migration, proliferation and glycolysis of pulmonary arterial smooth muscle cells: implications for pulmonary arterial hypertension.YULINK 在肺动脉平滑肌细胞迁移、增殖和糖酵解中的新作用：对肺动脉高压的影响。
Biol Res. 2023 Dec 7;56(1):66. doi: 10.1186/s40659-023-00480-z.
9
Plasminogen activator Inhibitor-2 inhibits pulmonary arterial smooth muscle cell proliferation in pulmonary arterial hypertension via PI3K/Akt and ERK signaling.纤溶酶原激活物抑制剂-2 通过 PI3K/Akt 和 ERK 信号通路抑制肺动脉高压中的肺动脉平滑肌细胞增殖。
Exp Cell Res. 2021 Jan 1;398(1):112392. doi: 10.1016/j.yexcr.2020.112392. Epub 2020 Nov 21.
10
High Shear Stress Reduces ERG Causing Endothelial-Mesenchymal Transition and Pulmonary Arterial Hypertension.高剪切应力降低ERG导致内皮-间充质转化和肺动脉高压。
Arterioscler Thromb Vasc Biol. 2025 Feb;45(2):218-237. doi: 10.1161/ATVBAHA.124.321092. Epub 2024 Dec 26.

本文引用的文献

1
Transcriptional profiles of pulmonary artery endothelial cells in pulmonary hypertension.肺动脉高血压中的肺动脉内皮细胞的转录组特征。
Sci Rep. 2023 Dec 18;13(1):22534. doi: 10.1038/s41598-023-48077-6.
2
Fabrication of a Gelatin-Based Microdevice for Vascular Cell Culture.用于血管细胞培养的明胶基微器件的制造
Micromachines (Basel). 2022 Dec 30;14(1):107. doi: 10.3390/mi14010107.
3
Pulmonary-arterial-hypertension (PAH)-on-a-chip: fabrication, validation and application.肺动脉高压（PAH）芯片：制作、验证和应用。
Lab Chip. 2020 Sep 21;20(18):3334-3345. doi: 10.1039/d0lc00605j. Epub 2020 Aug 4.
4
Nanoparticle-based Cell Trackers for Biomedical Applications.基于纳米粒子的细胞示踪剂在生物医学中的应用。
Theranostics. 2020 Jan 12;10(4):1923-1947. doi: 10.7150/thno.39915. eCollection 2020.
5
Collagen and gelatin.胶原蛋白和明胶。
Annu Rev Food Sci Technol. 2015;6:527-57. doi: 10.1146/annurev-food-031414-111800. Epub 2015 Mar 23.
6
Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping.用于前哨淋巴结定位的近红外荧光II型量子点
Nat Biotechnol. 2004 Jan;22(1):93-7. doi: 10.1038/nbt920. Epub 2003 Dec 7.