Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
Department of Veterinary Radiology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
PLoS One. 2024 Sep 19;19(9):e0310505. doi: 10.1371/journal.pone.0310505. eCollection 2024.
Chiari-like malformation in dogs and Chiari malformation type 1 in humans are conditions characterized by a relatively small caudal cranial fossa, leading to cerebellar herniation. This study aimed to develop a rat model of Chiari-like malformation using surgical techniques based on morphological characteristics observed in dogs.
Endocranial magnetic resonance images of both normal dogs and dogs diagnosed with Chiari-like malformation were retrospectively analyzed. Measurements of the caudal cranial fossa volume, rostral and medial fossa volume, and volume index were taken. The differences in caudal cranial fossa volume and volume index between normal dogs and those diagnosed with Chiari-like malformation were then utilized to create a rat model of Chiari-like malformation through surgical intervention. The measurements were conducted on both the rat Chiari-like malformation models and normal rats, with each measurement taken twice and the mean values calculated.
Significant differences were found between normal dogs and dogs diagnosed with Chiari-like malformation in terms of the volume of the caudal cranial fossa (27.62% reduction) and the volume index (23.36% reduction) (p<0.05). These differences were used to develop a rat model, which also showed significant reductions in both caudal cranial fossa volume (29.52%) and volume index (28.30%) compared to normal rats (p<0.05). The condition in the rat model was confirmed through magnetic resonance imaging, which revealed cerebellar herniation into the foramen magnum.
The study successfully established a rat model of Chiari-like malformation that accurately reproduces the morphological features observed in dogs. This model potentially serves as a valuable tool for investigating the pathological mechanisms and potential therapeutic approaches for Chiari-like malformation in veterinary medicine.
犬的 Chiari 样畸形和人类的 Chiari 畸形 I 型是一种特征为颅后窝相对较小，导致小脑疝出的疾病。本研究旨在基于犬中观察到的形态学特征，采用手术技术来建立犬 Chiari 样畸形的大鼠模型。
回顾性分析了正常犬和诊断为 Chiari 样畸形犬的颅内磁共振图像。测量颅后窝容积、颅前窝和颅中窝容积以及容积指数。然后，利用正常犬和 Chiari 样畸形犬的颅后窝容积和容积指数的差异，通过手术干预来建立 Chiari 样畸形大鼠模型。对大鼠 Chiari 样畸形模型和正常大鼠进行测量，每个测量重复两次，计算平均值。
正常犬和 Chiari 样畸形犬在颅后窝容积（减少 27.62%）和容积指数（减少 23.36%）方面存在显著差异（p<0.05）。这些差异用于建立大鼠模型，与正常大鼠相比，大鼠模型的颅后窝容积（减少 29.52%）和容积指数（减少 28.30%）也显著降低（p<0.05）。磁共振成像证实了大鼠模型的小脑疝出到枕骨大孔。
本研究成功建立了一种准确再现犬中观察到的形态学特征的大鼠 Chiari 样畸形模型。该模型可能成为兽医医学中研究 Chiari 样畸形病理机制和潜在治疗方法的有价值工具。
