Wang Xinran, Zang Xufeng, Zhou Hong, Wang Na, Fang Yishan, Cui Bo
School of Food Science and Engineering, State Key Laboratory of Biobased Material and Green Papermaking, School of Materials Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
College of Science, Huzhou University, Zhejiang, Huzhou 313000, China.
Food Chem. 2025 Jan 15;463(Pt 2):141294. doi: 10.1016/j.foodchem.2024.141294. Epub 2024 Sep 14.
Trichlorfon (TCF) has the possibility of contaminating agricultural crops and posing some health risks to humans. Herein, an electrochemiluminescence (ECL) sensor based on Fe, Cu dual-atom catalysts (Fe/Cu-N-C DACs) and Au@Luminol was developed for the ultrasensitive detection of TCF. Fe/Cu-N-C with diatomic sites has a very high catalytic activity and can be used as a co-reaction accelerator to activate HO to generate a large number of hydroxyl radicals which triggered a strong cathodic ECL signal of luminol. TCF molecularly imprinted polymer (MIP) was further introduced as a specific recognition element, and the interaction between the template molecule and the functional monomer was verified by molecular docking technique. The developed sensing platform was successfully applied to the ultrasensitive detection of TCF with a linear range from 1.0 pg/mL to 5.0 μg/mL with a low detection limit (0.39 pg/mL). This study broadens the application of DACs in ECL sensing.
敌百虫（TCF）有可能污染农作物并对人类构成一些健康风险。在此，开发了一种基于铁、铜双原子催化剂（Fe/Cu-N-C DACs）和金@鲁米诺的电化学发光（ECL）传感器，用于超灵敏检测TCF。具有双原子位点的Fe/Cu-N-C具有非常高的催化活性，可作为共反应促进剂激活HO以产生大量羟基自由基，从而引发鲁米诺强烈的阴极ECL信号。进一步引入TCF分子印迹聚合物（MIP）作为特异性识别元件，并通过分子对接技术验证了模板分子与功能单体之间的相互作用。所开发的传感平台成功应用于TCF的超灵敏检测，线性范围为1.0 pg/mL至5.0 μg/mL，检测限低（0.39 pg/mL）。本研究拓宽了DACs在ECL传感中的应用。
