College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Integrated Scientific Research Base of Edible Fungi Processing and Comprehensive Utilization Technology, Ministry of Agriculture and Rural Affairs, Fuzhou 350002, China; Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Integrated Scientific Research Base of Edible Fungi Processing and Comprehensive Utilization Technology, Ministry of Agriculture and Rural Affairs, Fuzhou 350002, China; Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China.
Int J Biol Macromol. 2024 Nov;280(Pt 2):135766. doi: 10.1016/j.ijbiomac.2024.135766. Epub 2024 Sep 17.
Tremella fuciformis (TF) is a mushroom with rich nutritional and medicinal value. This study aimed to develop an efficient extraction technique for TF polysaccharide (TFP) to enhance its health benefits. TF was subjected to steam explosion (SE) pretreatment at 0.5, 1.0, and 1.5 MPa for 60 s, followed by polysaccharide extraction. The extraction yield of TFP increased from 15.42 % to 50.16 % at 1.0 MPa. SE disrupted the dense structure of TFP, significantly improving total sugar and uronic acid contents, monosaccharide molar percentages of mannose and glucose, specific surface area, and ζ potential by 0.16, 0.4, 0.01, 0.83, 0.19, and 0.26 times at 0.5 MPa (P < 0.05). With increasing SE pressure, the thermal stability of TFP was enhanced, while its elasticity, viscosity, molecular weight, and particle size were reduced. TFP at 0.5 MPa significantly extended the lifespan of Drosophila melanogaster, with T reaching 74 d for females and 60 d for males at a dosage of 0.015625 %, indicating a 0.32-fold enhancement. TFP enhanced climbing ability and antioxidant stress resistance, increased antioxidant enzyme activities and total antioxidant capacity, and reduced malondialdehyde levels, indicating its anti-aging effects. These findings provide theoretical and technical support for the high-value development and utilization of TFP.
银耳（TF）是一种具有丰富营养和药用价值的蘑菇。本研究旨在开发一种高效的 TF 多糖（TFP）提取技术，以增强其健康益处。将 TF 进行蒸汽爆破（SE）预处理，压力分别为 0.5、1.0 和 1.5 MPa，时间为 60 s，然后进行多糖提取。在 1.0 MPa 下，TFP 的提取率从 15.42%提高到 50.16%。SE 破坏了 TFP 的致密结构，使总糖和糖醛酸含量、甘露糖和葡萄糖的单糖摩尔百分比、比表面积和 ζ 电位分别显著提高了 0.16、0.4、0.01、0.83、0.19 和 0.26 倍（P<0.05）。随着 SE 压力的增加，TFP 的热稳定性增强，而其弹性、粘度、分子量和粒径减小。在 0.5 MPa 下的 TFP 显著延长了黑腹果蝇的寿命，雌性的 T 达到 74 天，雄性的 T 达到 60 天，剂量为 0.015625%，提高了 0.32 倍。TFP 增强了果蝇的攀爬能力和抗氧化应激能力，增加了抗氧化酶活性和总抗氧化能力，降低了丙二醛水平，表明其具有抗衰老作用。这些发现为 TFP 的高值化开发利用提供了理论和技术支持。
