非热大气压气体放电等离子体促进兔模型中肌腱-骨结合部修复。
Non-thermal atmospheric pressure gas discharge plasma enhances tendon-to-bone junction repair in a rabbit model.
作者信息
Nakazawa Katsumasa, Toyoda Hiromitsu, Manaka Tomoya, Orita Kumi, Hirakawa Yoshihiro, Ito Yoichi, Saito Kosuke, Iio Ryosuke, Ban Yoshitaka, Yao Hana, Kobayashi Yuto, Oh Jun-Seok, Shirafuji Tatsuru, Nakamura Hiroaki
机构信息
Department of Orthopedic Surgery, Graduate School of Medicine, Osaka City University, Osaka, Japan.
Department of Orthopedic Surgery, Graduate School of Medicine, Osaka City University, Osaka, Japan; Department of Orthopedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan.
出版信息
J Shoulder Elbow Surg. 2025 Apr;34(4):918-927. doi: 10.1016/j.jse.2024.07.039. Epub 2024 Sep 17.
BACKGROUND
Plasma is a collection of active particles generated by dissociating molecules and ionizing atoms through applying high energy to a gas, such as high-sound heating or electrical shock. Recently, many reports have been published on the effectiveness of non-thermal atmospheric pressure gas discharge plasma (NTAPP) on living organisms. Furthermore, we have reported on the promotion of bone and tendon repair by NTAPP irradiation. We hypothesized that irradiation of NTAPP would promote the repair of the tendon-bone junction in a rotator cuff repair. This study investigated the effect of NTAPP irradiation on the healing process of the tendon-bone junction.
METHODS
Among 36 Japanese white rabbits, the infraspinatus tendon was detached from the humeral insertion site. A 3.2 mm bone tunnel was then created at the original insertion site of the infraspinatus muscle. The left shoulder was irradiated with NTAPP at a distance of 1 cm from the bone tunnel for 5 minutes (plasma-treated group), while the right shoulder was not irradiated (control group). The rabbits were sacrificed at 2, 4, and 8 weeks postoperatively, and 6 of each were used for histological evaluation. Mechanical tests were also performed on 6 specimens each at 4 and 8 weeks.
RESULTS
Histological evaluation showed that at 4 weeks, the histological tendon to bone maturing score was 6.8 ± 1.3 in the plasma-treated group and 4.8 ± 1.6 in the control group (P < .01); at 8 weeks it was 9.0 ± 1.0 in the plasma-treated group and 5.2 ± 1.1 in the control group (P < .01). Fibrocartilage formation and new bone formation were observed at both 4 and 8 weeks. In the mechanical test, the plasma-treated group had 75.0 ± 18.9 N in ultimate load to failure at 8 weeks. In the control group, it was 51.1 ± 7.9 N. (P = .04).
CONCLUSION
The repair of the rotator cuff at the tendon-bone junction was significantly improved at 4 and 8 weeks by irradiation with NTAPP.
背景
等离子体是通过对气体施加高能，如高声加热或电击，使分子解离和原子电离而产生的活性粒子集合。最近，关于非热大气压气体放电等离子体（NTAPP）对生物体有效性的报道很多。此外，我们报道了NTAPP照射可促进骨和肌腱修复。我们假设NTAPP照射会促进肩袖修复中肌腱-骨结合处的修复。本研究调查了NTAPP照射对肌腱-骨结合处愈合过程的影响。
方法
在36只日本白兔中，将冈下肌腱从肱骨附着点处分离。然后在冈下肌的原附着点处制作一个3.2毫米的骨隧道。左侧肩部在距离骨隧道1厘米处用NTAPP照射5分钟（等离子体治疗组），而右侧肩部不进行照射（对照组）。术后2周、4周和8周处死兔子，每组各取6只用于组织学评估。在4周和8周时，每组还分别对6个标本进行了力学测试。
结果
组织学评估显示，在4周时，等离子体治疗组的组织学肌腱到骨成熟评分是6.8±1.3，对照组是4.8±1.6（P<.01）；在8周时，等离子体治疗组是9.0±1.0，对照组是5.2±1.1（P<.01）。在4周和8周时均观察到纤维软骨形成和新骨形成。在力学测试中, 血浆治疗组在8周时的极限破坏载荷为75.0±18.9 N。对照组为51.1±7.9 N（P = 0.04）。
结论
NTAPP照射在4周和8周时显著改善了肩袖肌腱-骨结合处的修复。
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