发现真菌基因组中的隐藏功能。

Discovering the hidden function in fungal genomes.

机构信息

Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada.

出版信息

Nat Commun. 2024 Sep 19;15(1):8219. doi: 10.1038/s41467-024-52568-z.

DOI:10.1038/s41467-024-52568-z

PMID:39300175

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11413187/

Abstract

New molecular technologies have helped unveil previously unexplored facets of the genome beyond the canonical proteome, including microproteins and short ORFs, products of alternative splicing, regulatory non-coding RNAs, as well as transposable elements, cis-regulatory DNA, and other highly repetitive regions of DNA. In this Review, we highlight what is known about this 'hidden genome' within the fungal kingdom. Using well-established model systems as a contextual framework, we describe key elements of this hidden genome in diverse fungal species, and explore how these factors perform critical functions in regulating fungal metabolism, stress tolerance, and pathogenesis. Finally, we discuss new technologies that may be adapted to further characterize the hidden genome in fungi.

摘要

新的分子技术帮助揭示了基因组中除了规范的蛋白质组之外的以前未被探索的方面，包括微蛋白和短开放阅读框，可变剪接的产物，调控非编码 RNA，以及转座元件、顺式调控 DNA 和 DNA 的其他高度重复区域。在这篇综述中，我们强调了真菌王国中这个“隐藏基因组”的已知内容。我们使用成熟的模式系统作为上下文框架，描述了不同真菌物种中这个隐藏基因组的关键要素，并探讨了这些因素如何在调节真菌代谢、应激耐受和发病机制中发挥关键作用。最后，我们讨论了可能被用于进一步描绘真菌中隐藏基因组的新技术。