Central European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech Republic.
Faculty of Medicine, Charles University, Pilsen, Czech Republic.
BMC Genomics. 2024 Sep 17;25(1):870. doi: 10.1186/s12864-024-10780-7.
Wastewaters are considered as important players in the spread of antimicrobial resistance, thus affecting the health of humans and animals. Here, we focused on wastewaters as a possible source of carbapenemase-producing Enterobacterales for the environment.
A total of 180 presumptive coliforms from hospital and municipal wastewaters, and a river in the Czech Republic were obtained by selective cultivation on meropenem-supplemented media and tested for presence of carbapenemase-encoding genes by PCR. Strains carrying genes of interest were characterized by testing antimicrobial susceptibility, carbapenemase production and combination of short- and long- read whole-genome sequencing. The phylogenetic tree including publicly available genomes of Enterobacter asburiae was conducted using Prokka, Roary and RAxML.
Three VIM-producing Enterobacter asburiae isolates, members of the Enterobacter cloacae complex, were detected from hospital and municipal wastewaters, and the river. The bla gene was located within a class 1 integron that was carried by different F-type plasmids and one non-typeable plasmid. Furthermore, one of the isolates carried plasmid-borne colistin-resistance gene mcr-10, while in another isolate chromosomally located mcr-9 without colistin resistance phenotype was detected. In addition, the analysis of 685 publicly available E. asburiae genomes showed they frequently carry carbapenemase genes, highlighting the importance of this species in the emergence of resistance to last-line antibiotics.
Our findings pointed out the important contribution of hospital and community wastewaters in transmission of multi-drug resistant pathogens.
废水中被认为是抗生素耐药性传播的重要因素，从而影响人类和动物的健康。在这里，我们关注的是废水中可能是产碳青霉烯酶肠杆菌科的环境来源。
从捷克共和国的医院和市政废水中以及河流中总共获得了 180 株假定大肠菌群，通过在美罗培南补充培养基上选择性培养，并通过 PCR 检测碳青霉烯酶编码基因的存在来检测这些菌群。通过测试抗菌药物敏感性、碳青霉烯酶的产生以及短读和长读全基因组测序的组合，对携带感兴趣基因的菌株进行了特征描述。使用 Prokka、Roary 和 RAxML 构建了包含公开的阴沟肠杆菌基因组的 Enterobacter asburiae 系统发育树。
从医院和市政废水中以及河流中检测到了 3 株产 VIM 的阴沟肠杆菌，属于阴沟肠杆菌复合体。bla 基因位于 1 类整合子内，该整合子由不同的 F 型质粒和一个非可分型质粒携带。此外，其中一个分离株携带质粒介导的黏菌素耐药基因 mcr-10，而另一个分离株则在染色体上检测到 mcr-9，没有黏菌素耐药表型。此外，对 685 个公开的阴沟肠杆菌基因组的分析表明，它们经常携带碳青霉烯酶基因，这突出了该物种在出现最后一线抗生素耐药性方面的重要性。
我们的研究结果指出了医院和社区废水在传播多药耐药病原体方面的重要贡献。
