Majocha R E, Marotta C A, Benes F M
Can J Biochem Cell Biol. 1985 Jun;63(6):577-84. doi: 10.1139/o85-076.
Antibodies raised against the 200 000 neurofilament protein (NFP) of rat brain have been successfully applied to the staining of human postmortem cortex using a modified immunoperoxidase procedure. Human anterior cingulate cortex which has been in formaldehyde fixative for as long as 4 years shows extensive and reliable staining of axons and, to a lesser degree, apical dendrites of cortical neurons. The immunostaining of 200 000 NFP in human cortex has revealed cytoarchitectural details not generally visible with other more conventional neuroanatomical stains, particularly when counterstaining with cresyl violet is employed. Potential applications of this approach to the pattern analysis of human cortical cytoarchitecture in both health and disease are discussed.
用改良的免疫过氧化物酶方法，针对大鼠脑200 000神经丝蛋白（NFP）产生的抗体已成功应用于人类死后皮质的染色。在甲醛固定剂中保存长达4年的人类前扣带回皮质，显示出轴突以及程度较轻的皮质神经元顶树突有广泛且可靠的染色。人类皮质中200 000 NFP的免疫染色揭示了用其他更传统的神经解剖学染色通常不可见的细胞结构细节，特别是在用甲酚紫复染时。讨论了这种方法在健康和疾病状态下人类皮质细胞结构模式分析中的潜在应用。
