Cohen M C, Mecley M, Antonia S J, Picciano P T
Cell Immunol. 1985 Oct 15;95(2):247-57. doi: 10.1016/0008-8749(85)90312-0.
Tumor cells can adhere to endothelial cell monolayers in vitro. The kinetics of this reaction are rapid; 50% of maximal binding occurs by 30 min of incubation. In the case of the P815 mastocytoma, the maximal percentage of binding is approximately 70%, suggesting that there are both binding and nonbinding tumor cell populations. Binding is independent of tumor cell dose over a 200-fold range of cell concentrations. Lymphokine-containing preparations were found to markedly suppress the binding of either P815 mastocytoma or Ehrlich ascites cells to endothelium. This effect appeared to be due to both diminished attachment and enhanced dissociation. The activity is found in the same molecular weight range as tumor migration inhibition factor (TMIF), and is not found in preparations lacking TMIF activity. Thus, the factor may prove to be TMIF itself or a lymphokine related to it. Of equal interest is the possibility that it represents a previously undescribed factor.
肿瘤细胞在体外可黏附于内皮细胞单层。该反应动力学迅速；孵育30分钟时达到最大结合量的50%。以P815肥大细胞瘤为例，最大结合百分比约为70%，这表明存在结合型和非结合型肿瘤细胞群体。在200倍的细胞浓度范围内，结合与肿瘤细胞剂量无关。发现含淋巴因子的制剂可显著抑制P815肥大细胞瘤或艾氏腹水癌细胞与内皮细胞的结合。这种作用似乎是由于细胞黏附减少和解离增强所致。该活性物质的分子量范围与肿瘤迁移抑制因子（TMIF）相同，在缺乏TMIF活性的制剂中未发现。因此，该因子可能就是TMIF本身或与之相关的一种淋巴因子。同样有趣的是，它可能代表一种以前未描述过的因子。
