Mao Lingxiang, Gao Qing, Shen Yuxuan, Bao Chenxuan, Xiang Huayuan, Chen Qiaoqiao, Gao Qianqian, Huang Feng, He Wenyuan, Wang Jianjun
Department of Laboratory Medicine, Affiliated Kunshan Hospital of Jiangsu University, Kunshan, Jiangsu, China.
Medical College, Yangzhou University, Yangzhou, Jiangsu, China.
Front Microbiol. 2024 Sep 5;15:1430052. doi: 10.3389/fmicb.2024.1430052. eCollection 2024.
Previous studies demonstrated that EV71-infected cells secrete extracellular vesicles (EVs), facilitating the transfer of viral components to recipient cells and thereby promoting virus spread. Considering lipid signaling plays a crucial role in EVs-mediated cell-to-cell communication, we compared the lipid profile of EVs secreted from uninfected and EV71-infected cells (EVs-Mock and EVs-EV71) using the human rhabdomyosarcoma (RD) cell model. These two groups of EVs were purified by using size exclusion chromatography (SEC), respectively, and evaluated by transmission electron microscopy (TEM), nanoparticle tracking technology (NTA), and Western blotting (WB). In-depth lipidomic analysis of EVs identified 1705 lipid molecules belonging to 43 lipid classes. The data showed a significant increase in the lipid content of EVs after EV71 infection. Meanwhile, we deeply analyzed the changes in lipids and screened for lipid molecules with significant differences compared EVs-EV71 with EVs-Mock EVs. Altogether, we report the alterations in the lipid profile of EVs derived from RD-cells after EV71 infection, which may affect the function of the EVs in the recipient cells.
先前的研究表明，感染肠道病毒71型（EV71）的细胞会分泌细胞外囊泡（EVs），促进病毒成分向受体细胞的转移，从而促进病毒传播。鉴于脂质信号在EVs介导的细胞间通讯中起关键作用，我们使用人横纹肌肉瘤（RD）细胞模型比较了未感染和感染EV71的细胞（EVs-Mock和EVs-EV71）分泌的EVs的脂质谱。这两组EVs分别通过尺寸排阻色谱法（SEC）纯化，并通过透射电子显微镜（TEM）、纳米颗粒跟踪技术（NTA）和蛋白质免疫印迹法（WB）进行评估。对EVs的深入脂质组学分析鉴定出属于43种脂质类别的1705种脂质分子。数据显示，EV71感染后EVs的脂质含量显著增加。同时，我们深入分析了脂质的变化，并筛选出与EVs-Mock相比在EVs-EV71中具有显著差异的脂质分子。总之，我们报道了EV71感染后RD细胞来源的EVs脂质谱的变化，这可能会影响EVs在受体细胞中的功能。
