Chu Qingqing, Fu Xingxian, Zhao Juan, Li Yuxin, Liu Lina, Zhang Liuqin, Zhang Yujie, Guo Yifan, Pei Yan, Zhang Mi
College of Agronomy and Biotechnology, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing, 400715 P. R. China.
Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Southwest University, Chongqing, P. R. China.
Mol Breed. 2024 Sep 17;44(10):64. doi: 10.1007/s11032-024-01500-w. eCollection 2024 Oct.
Cotton is a widely planted commercial crop in the world. Enhancing fiber yield and quality is a long-term goal for cotton breeders. Our previous work has demonstrated that fine promotion of auxin biosynthesis in ovule epidermis, by overexpressing , has a significant improvement on lint yield and fiber fineness. Lately, transgenic cottons overexpressing variants modify mature fiber length by controlling GhPIN3a-mediated polar auxin transport in ovules. Here, this study showed that all these related cottons displayed unsatisfactory agronomic performance in field conditions. Yet extra auxin supply could promote their fiber development, suggesting inadequate auxin supply in the ovules. Thus, these cottons were integrated with enhanced auxin synthesis by crossing with cotton. All the transgene-stacked cottons exhibited synergetic effects on cotton yield (seedcotton yield, lint yield, and lint percentage) and quality (length, strength, and micronaire). Notably, comparing to the background, the transgene-stacked cotton co-expressing and (constitutively active ) exhibited a 12.6% increase in seedcotton yield and a 19.0% increase in lint yield over a three-year field trial, and simultaneously resulted in further improvement on fiber length, strength, and micronaire. Collectively, our data provide a potential strategy for genetic improvement on cotton fiber yield and quality.
The online version contains supplementary material available at 10.1007/s11032-024-01500-w.
棉花是世界上广泛种植的经济作物。提高纤维产量和质量是棉花育种者的长期目标。我们之前的研究表明，通过过表达在胚珠表皮中精细促进生长素生物合成，对皮棉产量和纤维细度有显著改善。最近，过表达变体的转基因棉花通过控制胚珠中GhPIN3a介导的生长素极性运输来改变成熟纤维长度。在此，本研究表明，所有这些相关棉花在田间条件下的农艺性能都不尽人意。然而，额外供应生长素可以促进它们的纤维发育，这表明胚珠中生长素供应不足。因此，通过与棉花杂交，将这些棉花与增强的生长素合成整合在一起。所有转基因叠加的棉花在棉花产量（籽棉产量、皮棉产量和皮棉百分比）和质量（长度、强度和马克隆值）方面都表现出协同效应。值得注意的是，与背景相比，在为期三年的田间试验中，共表达和（组成型活性）的转基因叠加棉花籽棉产量提高了12.6%，皮棉产量提高了19.0%，同时纤维长度、强度和马克隆值也得到了进一步改善。总体而言，我们的数据为棉花纤维产量和质量的遗传改良提供了一种潜在策略。
在线版本包含可在10.1007/s11032-024-01500-w获取的补充材料。
