Zang Yihao, Hu Yan, Xu Chenyu, Wu Shenjie, Wang Yangkun, Ning Zhiyuan, Han Zegang, Si Zhanfeng, Shen Weijuan, Zhang Yayao, Fang Lei, Zhang TianZhen
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
iScience. 2021 Jul 30;24(8):102930. doi: 10.1016/j.isci.2021.102930. eCollection 2021 Aug 20.
Cotton fiber is an excellent model for studying plant cell elongation and cell wall biogenesis as well because they are highly polarized and use conserved polarized diffuse growth mechanism. Fiber strength is an important trait among cotton fiber qualities due to ongoing changes in spinning technology. However, the molecular mechanism of fiber strength forming is obscure. Through map-based cloning, we identified the fiber strength gene . Increasing its expression, the fiber strength of the transgenic cotton was significantly enhanced compared to the receptor W0 and the helices number of the transgenic fiber was remarkably increased. Additionally, we proved that GhUBX regulates the fiber helical growth by degrading the GhSPL1 via the ubiquitin 26S-proteasome pathway. Taken together, we revealed the internal relationship between fiber helices and fiber stronger. It will be useful for improving the fiber quality in cotton breeding and illustrating the molecular mechanism for plant twisted growth.
棉纤维也是研究植物细胞伸长和细胞壁生物合成的优秀模型，因为它们高度极化并采用保守的极化扩散生长机制。由于纺纱技术的不断变化，纤维强度是棉纤维品质中的一个重要性状。然而，纤维强度形成的分子机制尚不清楚。通过图位克隆，我们鉴定出了纤维强度基因。增加其表达后，与受体W0相比，转基因棉花的纤维强度显著提高，且转基因纤维的螺旋数明显增加。此外，我们证明了GhUBX通过泛素26S蛋白酶体途径降解GhSPL1来调节纤维螺旋生长。综上所述，我们揭示了纤维螺旋与纤维强度之间的内在关系。这将有助于在棉花育种中提高纤维品质，并阐明植物扭曲生长的分子机制。
