Epidemiology and Modelling of Antibiotic Evasion Unit, Institut Pasteur, 75475, Paris, France.
Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
Eur J Clin Microbiol Infect Dis. 2024 Dec;43(12):2277-2292. doi: 10.1007/s10096-024-04943-3. Epub 2024 Sep 20.
Non-optimal vaginal microbiota lacking lactobacilli and comprising a wide array of anaerobic bacteria, typified by community state type (CST) IV, have been associated with adverse gynecological and pregnancy outcomes. Here, we investigate the stability of the vaginal microbiota sampled every 6 months over 18 months and how samples distantly collected combined with exposures could provide insight on future microbiota compositional changes.
Vaginal microbiota dynamics were analyzed in 241 female students aged 18-24 years and negative for Chlamydia trachomatis and Neisseria gonorrhoeae. The vaginal microbiota was characterized using 16S rRNA gene amplicon sequencing and assigned to CSTs. Vaginal microbiota longitudinal profiles were determined through hierarchical clustering.
At baseline, 11.2% of participants had a CST IV, 40.5% a CST I (Lactobacillus crispatus-dominated), and 38.1% a CST III (Lactobacillus iners-dominated). A total of 345 CST transitions were observed over the study period. Pain during sexual intercourse was associated with a higher probability of transition from CST III to CST IV, while self-reported yeast infection was associated with a higher probability of transition from CST IV to CST I. Over the study period, 32.0% participants displayed a stable CST trajectory. Composition of the vaginal microbiota of a single sample predicted with good accuracy the CST trajectory over the following 18 months.
Vaginal longitudinal CST patterns over 18 months could be clustered into three main groups of trajectories. Performing molecular characterization at a single time point could contribute to improved preventive care and optimization of young women's reproductive and sexual health.
gov Identifier: NCT02904811. Registration date: September 19, 2016.
缺乏乳杆菌且由多种厌氧菌组成的非最佳阴道微生物群，以社区状态类型（CST）IV 为代表，与不良妇科和妊娠结局相关。在这里，我们研究了阴道微生物群在 18 个月内每 6 个月采样一次的稳定性，以及如何结合远距离采集的样本和暴露情况来洞察未来的微生物群组成变化。
对 241 名年龄在 18-24 岁之间且沙眼衣原体和淋病奈瑟菌均为阴性的女学生的阴道微生物群动态进行了分析。使用 16S rRNA 基因扩增子测序对阴道微生物群进行了表征，并将其分配到 CST 中。通过层次聚类确定阴道微生物群纵向分布。
在基线时，11.2%的参与者有 CST IV，40.5%有 CST I（以乳杆菌脆明为主导），38.1%有 CST III（以乳杆菌内氏为主导）。在研究期间共观察到 345 次 CST 转换。性交时疼痛与从 CST III 向 CST IV 转变的可能性更高相关，而自述酵母菌感染与从 CST IV 向 CST I 转变的可能性更高相关。在研究期间，32.0%的参与者显示出稳定的 CST 轨迹。单个样本的阴道微生物群组成可以很好地预测接下来 18 个月的 CST 轨迹。
18 个月内阴道纵向 CST 模式可聚类为三个主要轨迹组。在单个时间点进行分子特征分析有助于改善年轻女性的生殖和性健康的预防保健和优化。
gov 标识符：NCT02904811。注册日期：2016 年 9 月 19 日。
