Janecki A, Jakubowiak A, Lukaszyk A
Mol Cell Endocrinol. 1985 Oct;42(3):235-43. doi: 10.1016/0303-7207(85)90054-1.
We investigated the influence of media from nonstimulated (SCCM) and FSH-stimulated (F-SCCM) cultured rat Sertoli cells on testosterone secretion by purified rat Leydig cells maintained in culture for 4 days. Both SCCM and F-SCCM stimulated Leydig cell secretory activity to a level 2-6 times that of the control, the effect being always maximal on day 3 of culture. On day 3, concentrated SCCM had a greater stimulatory effect on testosterone secretion than the original (i.e. non-concentrated) one, the effect being dose-related and similar to that exerted by concentrated F-SCCM. On the other hand, original as well as concentrated F-SCCM stimulated the basal testosterone secretion in a dose-dependent manner on day 1 to about 200% and 400% of the control level, respectively, whereas SCCM exerted the 'early' effect only as a concentrated preparation. Preincubation of Leydig cells with F-SCCM enhanced both basal (190% control) and LH-stimulated (274% control) testosterone secretion when the LH (10 ng/ml) was added for 3 h on day 1. The enhanced influence of SCCM was noted only with the LH-stimulated cells (140% control). It is concluded that, in culture, Sertoli cells release at least 2 factors which enhance testosterone secretion by Leydig cells in vitro. One of them seems to be FSH-dependent and increases both basal and LH-stimulated testosterone secretion. This factor (MW greater than 1 kDa) is heat-labile and exerts its maximal effect between 12 and 18 h of culture. The second factor(s) acts predominantly on day 3 of culture, is apparently FSH-independent, and its influence on Leydig cell testosterone may be, at least in part, nonspecific.
我们研究了来自未受刺激（SCCM）和促卵泡激素刺激（F-SCCM）培养的大鼠支持细胞的培养基，对体外培养4天的纯化大鼠睾丸间质细胞睾酮分泌的影响。SCCM和F-SCCM均能将睾丸间质细胞的分泌活性刺激至对照水平的2至6倍，这种作用在培养第3天总是达到最大值。在第3天，浓缩的SCCM对睾酮分泌的刺激作用比原始（即未浓缩）的培养基更强，该作用呈剂量依赖性，且与浓缩的F-SCCM所产生的作用相似。另一方面，原始的以及浓缩的F-SCCM在第1天以剂量依赖性方式分别将基础睾酮分泌刺激至对照水平的约200%和400%，而SCCM仅作为浓缩制剂发挥“早期”作用。当在第1天加入促黄体生成素（LH，10 ng/ml）3小时时，用F-SCCM预孵育睾丸间质细胞可增强基础（为对照的190%）和LH刺激（为对照的274%）的睾酮分泌。仅在LH刺激的细胞中观察到SCCM增强的影响（为对照的140%）。结论是，在培养中，支持细胞释放至少2种因子，可在体外增强睾丸间质细胞的睾酮分泌。其中一种因子似乎依赖于促卵泡激素，可增加基础和LH刺激的睾酮分泌。该因子（分子量大于1 kDa）对热不稳定，在培养12至18小时发挥最大作用。第二种因子主要在培养第3天起作用，显然不依赖于促卵泡激素，其对睾丸间质细胞睾酮的影响可能至少部分是非特异性的。
