Chadwick C M, Collodi P R, Sussman M
Differentiation. 1985;29(2):101-8. doi: 10.1111/j.1432-0436.1985.tb00301.x.
During fruiting-body construction by Dictyostelium discoideum, the formation and subsequent maintenance of the multicellular assembly involve two stage-specific cohesive systems that are acquired sequentially and are distinguishable on serological and genetic grounds. We demonstrated that both systems, termed aggregation related (AR) and postaggregation related (PAR), can function in vitro. Ghosts prepared from cells of the wild-type and of a cohesion-defective mutant that were harvested during growth and at aggregation and postaggregative stages of fruiting-body construction exhibited the same cohesive properties as the cells from which they were derived. Membrane fragments prepared from the ghosts by mechanical disruption retained these cohesive properties.
在盘基网柄菌构建子实体的过程中，多细胞聚集体的形成及后续维持涉及两个阶段特异性的黏附系统，这两个系统是依次获得的，并且在血清学和遗传学方面可加以区分。我们证明，这两个系统，即聚集相关（AR）系统和聚集后相关（PAR）系统，均可在体外发挥作用。从野生型细胞以及一个黏附缺陷型突变体细胞制备的“幽灵细胞”，这些细胞分别是在生长阶段、子实体构建的聚集阶段和聚集后阶段收获的，它们表现出与来源细胞相同的黏附特性。通过机械破碎从“幽灵细胞”制备的膜片段保留了这些黏附特性。
