Perciballi M, Pintauro S J
Food Chem Toxicol. 1985 Aug;23(8):737-40. doi: 10.1016/0278-6915(85)90267-4.
Corn oil samples were heated, with aeration, to 210 degrees C for a total of 5 hr. Both fresh and oxidized samples were urea-fractionated and the individual fractions were administered to male Sprague-Dawley rats by gastric intubation. The effects of urea adducts, adduct-free fractions, non-saponifiable fractions and unfractioned fresh and thermally oxidized oil samples on hepatic, intestinal and colonic drug-metabolizing enzymes were determined. The treatments had no significant effects on hepatic or intestinal drug-metabolizing or mixed-function oxidase activities. There was a significant (P less than 0.05) increase in colonic UDP-glucuronyltransferase activity in rats treated with thermally oxidized corn oil, while the non-saponifiable fraction of the same sample decreased (P less than 0.1) the activity of this enzyme. There was also a significant increase in the activity of colonic benzo[a]pyrene hydroxylase in rats treated with the non-adduct fraction or with urea adducts of the thermally oxidized corn oil. These data suggest the colon as a possible specific site for the alteration of mixed-function oxidase activities by products of thermally oxidized oils.
玉米油样品在通气条件下加热至210摄氏度，共加热5小时。对新鲜样品和氧化样品都进行了尿素分级分离，并通过胃管将各个级分给予雄性斯普拉格-道利大鼠。测定了尿素加合物、无加合物级分、不皂化级分以及未分级的新鲜和热氧化油样品对肝脏、肠道和结肠药物代谢酶的影响。这些处理对肝脏或肠道药物代谢或混合功能氧化酶活性没有显著影响。用热氧化玉米油处理的大鼠结肠UDP-葡萄糖醛酸转移酶活性显著增加（P小于0.05），而同一样品的不皂化级分使该酶的活性降低（P小于0.1）。用热氧化玉米油的无加合物级分或尿素加合物处理的大鼠结肠苯并[a]芘羟化酶活性也显著增加。这些数据表明，结肠可能是热氧化油产物改变混合功能氧化酶活性的一个特定部位。
