利用基于 Cas9 的可接合载体对革兰氏阴性菌中的常见质粒进行修复。

Curing of common plasmids in gram-negative bacteria using a Cas9-based conjugative vector.

机构信息

Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ 07110, United States of America.

出版信息

J Microbiol Methods. 2024 Nov;226:107047. doi: 10.1016/j.mimet.2024.107047. Epub 2024 Sep 18.

DOI:10.1016/j.mimet.2024.107047

Abstract

We report the creation of 17 Escherichia coli strains harboring the conjugative plasmid pLCasCureT with a CRISPR-Cas9 system to surgically "cure" the most common plasmids among Enterobacterales species. This approach can create isogenic pairs of strains to study host-plasmid interactions, correlate plasmid genotype and phenotype, and create plasmid-free cloning strains.

摘要

我们报告了 17 株携带可移动质粒 pLCasCureT 的大肠杆菌菌株的创建，该质粒带有 CRISPR-Cas9 系统，用于对肠杆菌科中最常见的质粒进行“手术治疗”。这种方法可以创建具有相同遗传背景的菌株对，用于研究宿主-质粒相互作用、关联质粒基因型和表型，并创建无质粒克隆菌株。