Xu Yang, Chen Jinxia, Li Yuxia, Sun Dejuan, Li Hua, Chen Lixia
Wuya College of Innovation, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Wuya College of Innovation, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, 110016, China; Institute of Structural Pharmacology & TCM Chemical Biology, Fujian Key Laboratory of Chinese Materia Medica, College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
Phytochemistry. 2025 Jan;229:114288. doi: 10.1016/j.phytochem.2024.114288. Epub 2024 Sep 19.
Sophora flavescens Aiton is a plant in the Leguminosae family. As a traditional Chinese medicine, it is used to treat eczema, bloody stool, skin pruritus, and so on. By studying non-alkaloid components in the roots of S. flavescens, we obtained a total of 49 compounds (1-49), including three undescribed flavonoids (13, 15 and 18), five undescribed isopentenyl flavonoids (32, 34, 38, 39 and 48), two known coumarins (1-2), three phenolic acids (3-5), one known isopentenyl flavonoids (19-31, 33, 35-37, 40-47 and 49). On the basis of chemical evidences and spectral data analysis (UV, ECD, Optical rotation data, 1D/2D-NMR and HR-ESI-MS), the structures of undescribed compounds were elucidated. The inhibitory effect of compounds 1-49 on LPS induced NO production in RAW 264.7 cells was detected. Compounds 11, 19, 21-24, and 28-30 showed significant inhibitory effects, and the IC values of compounds 11 and 22 even reached 4.58 ± 0.66 and 4.53 ± 0.66 μM. This study suggests that flavonoids may be the main component that exerts anti-inflammatory effects in the non-alkaloid extraction layer of the extract from the roots of S. flavescens.
苦参是豆科植物。作为一种传统中药，它被用于治疗湿疹、便血、皮肤瘙痒等。通过研究苦参根中的非生物碱成分，我们总共获得了49种化合物（1 - 49），包括三种未描述的黄酮类化合物（13、15和18）、五种未描述的异戊烯基黄酮类化合物（32、34、38、39和48）、两种已知的香豆素（1 - 2）、三种酚酸（3 - 5）、一种已知的异戊烯基黄酮类化合物（19 - 31、33、35 - 37、40 - 47和49）。基于化学证据和光谱数据分析（紫外光谱、电子圆二色谱、旋光数据、一维/二维核磁共振和高分辨电喷雾电离质谱），阐明了未描述化合物的结构。检测了化合物1 - 49对脂多糖诱导的RAW 264.7细胞中一氧化氮产生的抑制作用。化合物11、19、21 - 24以及28 - 30显示出显著的抑制作用，化合物11和22的半数抑制浓度值甚至达到4.58±0.66和4.53±0.66μM。本研究表明，黄酮类化合物可能是苦参根提取物非生物碱提取层中发挥抗炎作用的主要成分。
