同种免疫诱导的胸腺细胞前列腺素水平变化：诱导生物活性的信号。

Alloimmunization-induced changes in thymocytes' prostaglandin levels: a signal for the induction of biological activity.

作者信息

Borda E, Genaro A, Peredo H, Cremaschi G, Leiros C P, Sterin-Borda L

出版信息

Int J Immunopharmacol. 1985;7(5):647-53. doi: 10.1016/0192-0561(85)90148-1.

Abstract

The effect of BALB/c anti C3H non-adherent thymocytes on the spontaneous activity of isolated C3H mouse atria was studied. Immune non-adherent thymocytes alone induced negative inotropic action, while macrophages alone did not have any effect. The same kind of collaborative effect between both cells is described. Cell-free supernatants obtained from non-adherent immune cells incubated with C3H myocardium induced the same biological activity than immune non-adherent thymocytes indicating that a soluble factor is involved. Inhibition of non-adherent thymocytes' cyclo-oxygenase activity prevented the negative inotropic effect of immune cells or cell-free supernatants. In contrast, when the inhibitors were applied upon myocardium did not modify the response. Supernatants from immune thymocytes plus myocardium, release higher amount of PGE series than those from non-immune thymocytes. It is proposed that non-adherent thymocytes are able to synthesize and release PGE series upon recognition of alloantigens expressed by atria cells; which in turn triggers the negative inotropic effect.

摘要

研究了BALB/c抗C3H非黏附性胸腺细胞对分离的C3H小鼠心房自发活动的影响。单独的免疫非黏附性胸腺细胞可诱导负性肌力作用，而单独的巨噬细胞则无任何作用。描述了两种细胞之间的同样的协同作用。用C3H心肌孵育非黏附性免疫细胞获得的无细胞上清液诱导出与免疫非黏附性胸腺细胞相同的生物学活性，表明涉及一种可溶性因子。抑制非黏附性胸腺细胞的环氧化酶活性可阻止免疫细胞或无细胞上清液的负性肌力作用。相反，当抑制剂作用于心肌时，并未改变反应。免疫胸腺细胞加心肌的上清液比非免疫胸腺细胞的上清液释放出更多的前列腺素E系列。有人提出，非黏附性胸腺细胞在识别心房细胞表达的同种异体抗原后能够合成并释放前列腺素E系列；这反过来又引发负性肌力作用。