海洋盐杆菌属（Salinirarus）新属，新种，盐沼盐球菌（Haloplanus salilacus）新种，海洋盐球菌（Haloplanus pelagicus）新种，嗜盐古菌（Haloplanus halophilus）新种和嗜盐古菌（Haloplanus halobius）新种，从商业粗盐中分离出的嗜盐古菌，具有作为盐发酵食品发酵剂的潜力。

Salinirarus marinus gen. nov., sp. nov., Haloplanus salilacus sp. nov., Haloplanus pelagicus sp. nov., Haloplanus halophilus sp. nov., and Haloplanus halobius sp. nov., halophilic archaea isolated from commercial coarse salts with potential as starter cultures for salt-fermented foods.

机构信息

School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang 212013, People's Republic of China.

出版信息

FEMS Microbiol Lett. 2024 Jan 9;371. doi: 10.1093/femsle/fnae075.

DOI:10.1093/femsle/fnae075

PMID:39304533

Abstract

Five halophilic archaeal strains, XH8T, CK5-1T, GDY1T, HW8-1T, and XH21T, were isolated from commercial coarse salt produced in different regions of China. Their 16S rRNA and rpoB' gene sequences indicated that four of the strains (CK5-1T, GDY1T, HW8-1T, and XH21T) represent distinct species within the genus Haloplanus (family Haloferacaceae), while strain XH8T represents a novel genus within the same family. These assignments were supported by phylogenetic and phylogenomic analyses, which showed that strains CK5-1T, GDY1T, HW8-1T, and XH21T cluster with the current species of the genus Haloplanus, while strain XH8T forms a separate branch from the genus Haloplanus. The digital DNA-DNA hybridization and average amino acid identity (AAI) values among these four strains and the current members of the genus Haloplanus were 23.1%-35.2% and 75.9%-83.8%, respectively; and those values between strain XH8T and other genera in the family Haloferacaceae were 18.8%-33.6% and 59.8%-66.6%, respectively, much lower than the threshold values for species demarcation. Strain XH8T may represent a novel genus of the family Haloferacaceae according to the cut-off value of AAI (≤72.1%) proposed to differentiate genera within the family Haloferacaceae. These five strains could be distinguished from the related species according to differential phenotypic characteristics. Based on these results, it is proposed that strain XH8T represents a novel genus within the family Haloferacaceae, and strains CK5-1T, GDY1T, HW8-1T, and XH21T represent four novel species of the genus Haloplanus, respectively. Additionally, these five strains possess genes encoding enzymes critical for the fermentation process in salt-fermented foods, indicating their potential as starter cultures for these applications.

摘要

从中国不同地区生产的商业粗盐中分离到 5 株嗜盐古菌菌株，分别为 XH8T、CK5-1T、GDY1T、HW8-1T 和 XH21T。它们的 16S rRNA 和 rpoB' 基因序列表明，其中 4 株（CK5-1T、GDY1T、HW8-1T 和 XH21T）代表 Haloferacaceae 科 Haloplanus 属中的不同种，而菌株 XH8T 代表同一科中的一个新属。这些分类是通过系统发育和系统基因组分析得到支持的，结果表明菌株 CK5-1T、GDY1T、HW8-1T 和 XH21T 与现有的 Haloplanus 属种聚类，而菌株 XH8T 则与 Haloplanus 属形成一个独立的分支。这 4 株菌与现有的 Haloplanus 属种之间的数字 DNA-DNA 杂交和平均氨基酸同一性（AAI）值分别为 23.1%-35.2%和 75.9%-83.8%；而菌株 XH8T 与 Haloferacaceae 科其他属种之间的数字 DNA-DNA 杂交和平均氨基酸同一性（AAI）值分别为 18.8%-33.6%和 59.8%-66.6%，均低于科内种的界定阈值。根据为区分 Haloferacaceae 科内属而提出的 AAI（≤72.1%）截断值，菌株 XH8T 可能代表该科的一个新属。这 5 株菌可根据表型特征的差异与相关种区分开来。基于这些结果，建议将菌株 XH8T 作为 Haloferacaceae 科内的一个新属，菌株 CK5-1T、GDY1T、HW8-1T 和 XH21T 分别代表 Haloplanus 属的四个新种。此外，这 5 株菌都拥有编码在盐发酵食品发酵过程中关键酶的基因，表明它们可能作为这些应用的起始培养物。