School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
Extremophiles. 2022 Aug 3;26(3):26. doi: 10.1007/s00792-022-01275-y.
Three halophilic archaeal strains, NEN8, GDY88 and ZY14, were isolated from a salt lake in Tibet and coarse sea salt samples from Guangdong and Hebei, China, respectively. These strains formed three separate clades (showing 94.4-95.8% and 87.1-89.4% similarities, respectively) and then clustered with the current Halorientalis members (showing 90.7-97.6% and 87.0-91.2% similarities, respectively), as revealed by phylogenetic analyses based on 16S rRNA and rpoB' genes. The overall genome-related index, average nucleotide identity (ANI), in silico DNA-DNA hybridization (DDH), average amino acid identity (AAI) and the percentage of conserved proteins (POCP) values, among the three strains and members of the genus Halorientalis were 76.0-88.0%, 21.3-37.2%, 69.0-88.3% and 57.7-78.1%, clearly below the threshold values for species demarcation. Strains NEN8, GDY88 and ZY14 could be distinguished from current Halorientalis species according to differential phenotypic characteristics. The major polar lipids of the three strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1) and disulfated mannosyl glucosyl diether (S-DGD). In addition, mannosyl glucosyl diether (DGD-1) was detected in strain NEN8 and phosphatidic acid (PA), posssulfated galactosyl mannosyl glucosyl diether (S-TGD-1) and sulfated mannosyl glucosyl diether-phosphatidic acid (S-DGD-PA) were observed in strain ZY14. These results revealed that strains NEN8 (= CGMCC 1.17213 = JCM 34155), GDY88 (= CGMCC 1.18548 = JCM 34481) and ZY14 (= CGMCC 1.17178 = JCM 34154) represent three novel species of the genus Halorientalis, for which the names Halorientalis salina sp. nov., Halorientalis marina sp. nov. and Halorientalis litorea sp. nov. are proposed.
从西藏盐湖、中国广东和河北粗盐样本中分别分离到 3 株嗜盐古菌 NEN8、GDY88 和 ZY14。这些菌株形成了 3 个独立的分支（分别显示出 94.4-95.8%和 87.1-89.4%的相似度），然后与现有的 Halorientalis 成员聚类（分别显示出 90.7-97.6%和 87.0-91.2%的相似度），这是基于 16S rRNA 和 rpoB' 基因的系统发育分析揭示的。3 株菌和 Halorientalis 属的成员之间的全基因组相关指数、平均核苷酸同一性（ANI）、体外 DNA-DNA 杂交（DDH）、平均氨基酸同一性（AAI）和保守蛋白百分比（POCP）值分别为 76.0-88.0%、21.3-37.2%、69.0-88.3%和 57.7-78.1%，明显低于种间划分的阈值。根据表型特征的差异，菌株 NEN8、GDY88 和 ZY14 可与现有的 Halorientalis 种区分开来。3 株菌的主要极性脂为磷脂酰甘油（PG）、磷脂酰甘油磷酸甲酯（PGP-Me）、硫酸化甘露糖基葡糖基二醚（S-DGD-1）和双硫酸化甘露糖基葡糖基二醚（S-DGD）。此外，菌株 NEN8 中检测到甘露糖基葡糖基二醚（DGD-1），菌株 ZY14 中检测到磷脂酸（PA）、硫酸化半乳糖基甘露糖基葡糖基二醚（S-TGD-1）和硫酸化甘露糖基葡糖基二醚-磷脂酸（S-DGD-PA）。这些结果表明，菌株 NEN8（=CGMCC 1.17213=JCM 34155）、GDY88（=CGMCC 1.18548=JCM 34481）和 ZY14（=CGMCC 1.17178=JCM 34154）代表了 Halorientalis 属的 3 个新种，分别命名为 Halorientalis salina sp. nov.、Halorientalis marina sp. nov. 和 Halorientalis litorea sp. nov.。
